Yoshitaka Muramatsu1, Takuya Matsui2, Masataka Deie3, Keiji Sato1. 1. Department of Orthopaedic Surgery, Aichi Medical University School of Medicine Nagakute, Aichi, Japan. 2. Department of Physiology, Aichi Medical University School of Medicine Nagakute, Aichi, Japan. 3. Department of Orthopaedic Surgery, Aichi Medical University School of Medicine Nagakute, Aichi, Japan snm3@aichi-med-u.ac.jp.
Abstract
AIM: We aimed to investigate the synergistic effects of pulsed electromagnetic field (PEMF) and doxorubicin therapy in a mouse osteosarcoma cell line (LM8 cells) in vitro. MATERIALS AND METHODS: The effects of PEMF (5 mT, 200 Hz) of different durations and doxorubicin on the proliferative activity of LM8 cells were measured by the MTT assay. Apoptotic-related factors such as cell-cycle phase, mitochondrial membrane potential, and caspase 3/7 activity were investigated using 4',6-diamidino-2-phenylindole staining and apoptosis kits. Identification of intracellular signaling molecules induced by the combination was comprehensively explored using a stress and apoptosis-related protein array kit. RESULTS: PEMF enhanced the inhibition of cell proliferation mediated by doxorubicin but did not affect the cell cycle, mitochondrial membrane potential, or doxorubicin-induced G2/M arrest. The combination of PEMF and doxorubicin altered a few signaling molecules. PEMF tended to reduce the doxorubicin-induced decrease of phosphorylated BAD, while reducing the increased expression of total IĸB and phosphorylated-CHK1 induced by doxorubicin. CONCLUSION: Our results indicate that combination of PEMF and doxorubicin could be a novel chemotherapeutic strategy. Copyright
AIM: We aimed to investigate the synergistic effects of pulsed electromagnetic field (PEMF) and doxorubicin therapy in a mouseosteosarcoma cell line (LM8 cells) in vitro. MATERIALS AND METHODS: The effects of PEMF (5 mT, 200 Hz) of different durations and doxorubicin on the proliferative activity of LM8 cells were measured by the MTT assay. Apoptotic-related factors such as cell-cycle phase, mitochondrial membrane potential, and caspase 3/7 activity were investigated using 4',6-diamidino-2-phenylindole staining and apoptosis kits. Identification of intracellular signaling molecules induced by the combination was comprehensively explored using a stress and apoptosis-related protein array kit. RESULTS:PEMF enhanced the inhibition of cell proliferation mediated by doxorubicin but did not affect the cell cycle, mitochondrial membrane potential, or doxorubicin-induced G2/M arrest. The combination of PEMF and doxorubicin altered a few signaling molecules. PEMF tended to reduce the doxorubicin-induced decrease of phosphorylated BAD, while reducing the increased expression of total IĸB and phosphorylated-CHK1 induced by doxorubicin. CONCLUSION: Our results indicate that combination of PEMF and doxorubicin could be a novel chemotherapeutic strategy. Copyright
Authors: J Kaszuba-Zwoinska; K Wojcik; M Bereta; A Ziomber; P Pierzchalski; E Rokita; J Marcinkiewicz; W Zaraska; P Thor Journal: J Physiol Pharmacol Date: 2010-04 Impact factor: 3.011
Authors: Mike Y Chen; Jing Li; Nianli Zhang; Erik I Waldorff; James T Ryaby; Philip Fedor; Yongsheng Jia; Yujun Wang Journal: Technol Cancer Res Treat Date: 2022 Jan-Dec