Mirian Saavedra Ururahy1, Fabiana Almeida Curylofo-Zotti1, Rodrigo Galo2, Lucas Fabricio Bahia Nogueira3, Ana Paula Ramos3, Silmara Aparecida Milori Corona4. 1. Department of Restorative Dentistry, Sao Paulo University, Café Avenue, Monte Alegre, Ribeirão Preto, SP, Brazil. 2. Department of Dentistry, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil. 3. Department of Chemistry, Sao Paulo University, Bandeirantes Avenue, Ribeirao Preto, SP, Brazil. 4. Department of Restorative Dentistry, Sao Paulo University, Café Avenue, Monte Alegre, Ribeirão Preto, SP, Brazil. Electronic address: silmaracorona@forp.usp.br.
Abstract
OBJECTIVE: To assess the effect of chitosan, at concentrations of 2.5% and 5.0%, on the wettability of the eroded dentin, followed by analysis of surface morphology by SEM. METHODS: 104 bovine dentin slabs were ground, polished and then immersed in 20mL of citric acid (pH=3.2) under continuous stirring for 2h. Specimens were randomly divided according to the dentin substrate: sound and eroded, and then, subdivided into 4 groups (n=10): without rewetting (control), 1% acetic acid, 2.5% chitosan and 5.0% chitosan. Then, a drop of the adhesive system Single Bond 2 (3M) was deposited onto surface of each specimen. The contact angle between dentin surface and the adhesive system was measured by using a goniometer. The other 24 specimens were subjected to analysis under SEM. Statistical analysis was performed using the normality test (Kolmogorov-Smirnov) and Analysis of Variance (ANOVA) (p>0.05). RESULTS: No differences were found between the angles produced on the eroded dentin rewetting with chitosan at the concentrations of 2.5% and 5%. CONCLUSION: The chitosan, regardless of the concentration used, did not influence the eroded dentin wettability. Through SEM analysis, it was found particles of chitosan deposited on the surface and within the dentinal tubules.
OBJECTIVE: To assess the effect of chitosan, at concentrations of 2.5% and 5.0%, on the wettability of the eroded dentin, followed by analysis of surface morphology by SEM. METHODS: 104 bovine dentin slabs were ground, polished and then immersed in 20mL of citric acid (pH=3.2) under continuous stirring for 2h. Specimens were randomly divided according to the dentin substrate: sound and eroded, and then, subdivided into 4 groups (n=10): without rewetting (control), 1% acetic acid, 2.5% chitosan and 5.0% chitosan. Then, a drop of the adhesive system Single Bond 2 (3M) was deposited onto surface of each specimen. The contact angle between dentin surface and the adhesive system was measured by using a goniometer. The other 24 specimens were subjected to analysis under SEM. Statistical analysis was performed using the normality test (Kolmogorov-Smirnov) and Analysis of Variance (ANOVA) (p>0.05). RESULTS: No differences were found between the angles produced on the eroded dentin rewetting with chitosan at the concentrations of 2.5% and 5%. CONCLUSION: The chitosan, regardless of the concentration used, did not influence the eroded dentin wettability. Through SEM analysis, it was found particles of chitosan deposited on the surface and within the dentinal tubules.
Authors: Madalena Belmar da Costa; António H S Delgado; Teresa Pinheiro de Melo; Tomás Amorim; Ana Mano Azul Journal: Biomater Investig Dent Date: 2021-02-15
Authors: Fernando Arias Alvarado; Maira Rivero Iriarte; Freddy Jordan Mariño; Sara Quijano-Guauque; León D Pérez; Yolima Baena; Claudia García-Guerrero Journal: Int J Biomater Date: 2021-10-31
Authors: Letícia Pena Botelho; Simone Gomes Dias De Oliveira; Dhelfeson Willya Douglas De Oliveira; Rodrigo Galo Journal: J Conserv Dent Date: 2022-06-13