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Literature DB >> 28060410

Preparation of Cell Cultures and Vaccinia Virus Stocks.

Catherine A Cotter¹, Patricia L Earl¹, Linda S Wyatt¹, Bernard Moss¹.

Abstract

The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.

Entities: Disease Species

Keywords: cell culture; modified vaccinia virus Ankara; vaccinia virus

Mesh：

Substances：
DNA, Viral

Year: 2017 PMID： 28060410 DOI： 10.1002/cpmb.33

Source DB: PubMed Journal: Curr Protoc Mol Biol ISSN： 1934-3647

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Cited

9 in total

1. Specific Anchoring and Local Translation of Poxviral ATI mRNA at Cytoplasmic Inclusion Bodies.

Authors: George C Katsafanas; Bernard Moss
Journal: J Virol Date: 2020-01-31 Impact factor: 5.103

2. Deletion of the Vaccinia Virus I2 Protein Interrupts Virion Morphogenesis, Leading to Retention of the Scaffold Protein and Mislocalization of Membrane-Associated Entry Proteins.

Authors: Seong-In Hyun; Andrea Weisberg; Bernard Moss
Journal: J Virol Date: 2017-07-12 Impact factor: 5.103

3. Identification of Vaccinia Virus Replisome and Transcriptome Proteins by Isolation of Proteins on Nascent DNA Coupled with Mass Spectrometry.

Authors: Tatiana G Senkevich; George C Katsafanas; Andrea Weisberg; Lisa R Olano; Bernard Moss
Journal: J Virol Date: 2017-09-12 Impact factor: 5.103

Preparation of Cell Cultures and Vaccinia Virus Stocks.

1. Specific Anchoring and Local Translation of Poxviral ATI mRNA at Cytoplasmic Inclusion Bodies.

2. Deletion of the Vaccinia Virus I2 Protein Interrupts Virion Morphogenesis, Leading to Retention of the Scaffold Protein and Mislocalization of Membrane-Associated Entry Proteins.

3. Identification of Vaccinia Virus Replisome and Transcriptome Proteins by Isolation of Proteins on Nascent DNA Coupled with Mass Spectrometry.

4. Identification of Poxvirus Genome Uncoating and DNA Replication Factors with Mutually Redundant Roles.

5. Early type I IFN blockade improves the efficacy of viral vaccines.

6. Modified vaccinia Ankara expressing EEHV1A glycoprotein B elicits humoral and cell-mediated immune responses in mice.

7. Expression of NAC1 Restrains the Memory Formation of CD8⁺ T Cells during Viral Infection.

8. LINE-1 retrotransposons facilitate horizontal gene transfer into poxviruses.

9. Vaccinia virus and Cowpox virus are not susceptible to the interferon-induced antiviral protein MxA.