Literature DB >> 28060405

Generation of Recombinant Vaccinia Viruses.

Linda S Wyatt1, Patricia L Earl1, Bernard Moss1.   

Abstract

This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are described. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented. © 2017 by John Wiley & Sons, Inc.
Copyright © 2017 John Wiley & Sons, Inc.

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Mesh：

Year:  2017        PMID： 28060405     DOI： 10.1002/cpmb.32

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  8 in total

1.  Identification of Poxvirus Genome Uncoating and DNA Replication Factors with Mutually Redundant Roles.

Authors:  Baoming Liu; Debasis Panda; Jorge D Mendez-Rios; Sundar Ganesan; Linda S Wyatt; Bernard Moss
Journal:  J Virol       Date:  2018-03-14       Impact factor: 5.103

Review 2.  CRISPR/Cas9-Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development.

Authors:  Arinze Okoli; Malachy I Okeke; Morten Tryland; Ugo Moens
Journal:  Viruses       Date:  2018-01-22       Impact factor: 5.048

3.  Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies.

Authors:  Chaojie Zhong; Hongjie Xia; Awadalkareem Adam; Binbin Wang; Renee L Hajnik; Yuejin Liang; Grace H Rafael; Jing Zou; Xiaofang Wang; Jiaren Sun; Lynn Soong; Alan D T Barrett; Scott C Weaver; Pei-Yong Shi; Tian Wang; Haitao Hu
Journal:  NPJ Vaccines       Date:  2021-11-29       Impact factor: 9.399

4.  Modified vaccinia Ankara expressing EEHV1A glycoprotein B elicits humoral and cell-mediated immune responses in mice.

Authors:  Taylor Pursell; Jennifer L Spencer Clinton; Jie Tan; Rongsheng Peng; Paul D Ling
Journal:  PLoS One       Date:  2022-03-21       Impact factor: 3.240

5.  Interferon α/β Decoy Receptor Encoded by a Variant in the Dryvax Smallpox Vaccine Contributes to Virulence and Correlates with Severe Vaccine Side Effects.

Authors:  Ruikang Liu; Jeffrey L Americo; Patricia L Earl; Jack Villani; Catherine A Cotter; Bernard Moss
Journal:  mBio       Date:  2022-02-22       Impact factor: 7.867

Review 6.  Poxvirus Recombination.

Authors:  David Hugh Evans
Journal:  Pathogens       Date:  2022-08-09

7.  Zinc-finger antiviral protein (ZAP) is a restriction factor for replication of modified vaccinia virus Ankara (MVA) in human cells.

Authors:  Chen Peng; Linda S Wyatt; Shira G Glushakow-Smith; Madhu Lal-Nag; Andrea S Weisberg; Bernard Moss
Journal:  PLoS Pathog       Date:  2020-08-31       Impact factor: 6.823

8.  Antisense oligonucleotide inhibition of hepatitis C virus (HCV) gene expression in livers of mice infected with an HCV-vaccinia virus recombinant.

Authors:  H Zhang; R Hanecak; V Brown-Driver; R Azad; B Conklin; M C Fox; K P Anderson
Journal:  Antimicrob Agents Chemother       Date:  1999-02       Impact factor: 5.191
  8 in total

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