| Literature DB >> 28059579 |
Xin-Ee Tan1, Hui-Min Neoh1,2, Mee-Lee Looi1,3, Siok Fong Chin1, Longzhu Cui4, Keiichi Hiramatsu2, Salasawati Hussin5, Rahman Jamal1.
Abstract
Comparative proteomic profiling between 2 vancomycin-intermediate Staphylococcus aureus (VISA) strains, Mu50Ω-vraSm and Mu50Ω-vraSm-graRm, and vancomycin-susceptible S. aureus (VSSA) strain Mu50Ω revealed upregulated levels of catabolic ornithine carbamoyltransferase (ArcB) of the arginine catabolism pathway in VISA strains. Subsequent analyses showed that the VISA strains have higher levels of cellular ATP and ammonia, which are by-products of arginine catabolism, and displayed thicker cell walls. We postulate that elevated cytoplasmic ammonia and ATP molecules, resulting from activated arginine catabolism upon acquisition of vraS and graR mutations, are important requirements facilitating cell wall biosynthesis, thereby contributing to thickened cell wall and consequently reduced vancomycin susceptibility in VISA strains.Entities:
Keywords: ATP; Staphylococcus aureus de résistance intermédiaire à la vancomycine; ammonia; ammoniac; arginine catabolism; catabolisme de l’arginine; cell wall thickening; vancomycin-intermediate Staphylococcus aureus; épaississement de la paroi cellulaire
Mesh：
Substances：
Year: 2016 PMID： 28059579 DOI： 10.1139/cjm-2016-0439
Source DB: PubMed Journal: Can J Microbiol ISSN： 0008-4166 Impact factor: 2.419