Leire Méndez-Giménez1,2, Sara Becerril1,2, Rafael Moncada2,3, Víctor Valentí2,4, Secundino Fernández2,5, Beatriz Ramírez1,2, Victoria Catalán1,2, Javier Gómez-Ambrosi1,2, Graça Soveral6, María M Malagón2,7, Carlos Diéguez2,8, Amaia Rodríguez9,10, Gema Frühbeck1,2,11. 1. Metabolic Research Laboratory, Clínica Universidad de Navarra, IdiSNA, Irunlarrea 1, 31008, Pamplona, Spain. 2. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. 3. Department of Anesthesia, Clínica Universidad de Navarra, IdiSNA, Pamplona, Spain. 4. Department of Surgery, Clínica Universidad de Navarra, IdiSNA, Pamplona, Spain. 5. Department of Otorhinolaryngology, Clínica Universidad de Navarra, IdiSNA, Pamplona, Spain. 6. Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal. 7. Department of Cell Biology, Physiology, and Immunology, Instituto Maimónides de Investigación Biomédica (IMIBIC)/Reina Sofia University Hospital/University of Córdoba, Córdoba, Spain. 8. Department of Physiology, CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain. 9. Metabolic Research Laboratory, Clínica Universidad de Navarra, IdiSNA, Irunlarrea 1, 31008, Pamplona, Spain. arodmur@unav.es. 10. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. arodmur@unav.es. 11. Department of Endocrinology and Nutrition, Clínica Universidad de Navarra, IdiSNA, Pamplona, Spain.
Abstract
BACKGROUND: Gastric plication is a minimally invasive bariatric surgical procedure, where the greater curvature is plicated inside the gastric lumen. Our aims were to analyze the effectiveness of gastric plication on the resolution of obesity, impaired glucose tolerance, and fatty liver in an experimental model of diet-induced obesity (DIO) and to evaluate changes in glycerol metabolism, a key substrate for adiposity and gluconeogenesis, in adipose tissue and the liver. METHODS: Male Wistar DIO rats (n = 58) were subjected to surgical (sham operation and gastric plication) or dietary interventions [fed a normal diet (ND) or high-fat diet (HFD) or pair-fed to the amount of food eaten by gastric-plicated animals]. The expression of aquaglyceroporins (AQPs) in epididymal (EWAT) and subcutaneous (SCWAT) fat and the liver was analyzed by real-time PCR and Western blot. RESULTS: Gastric plication did not result in a significant weight loss in DIO rats, showing a modest reduction in whole-body adiposity and hepatic steatosis. However, gastric-plicated animals exhibited an improvement in basal glycemia and glucose clearance, without changes in hepatic gluconeogenic genes. DIO was associated with an increase in glycerol, higher AQP3 and AQP7 in EWAT and SCWAT, and a decrease in hepatic AQP9. Gastric plication downregulated AQP3 in both fat depots without changes in adipose AQP7 and hepatic AQP9. CONCLUSION: Gastric plication results in a modest reduction in adiposity and hepatosteatosis but restores glycemia by downregulating AQP3, which entails lower efflux of glycerol from fat, lower plasma glycerol availability, and a reduced use of glycerol as a substrate for hepatic gluconeogenesis.
BACKGROUND: Gastric plication is a minimally invasive bariatric surgical procedure, where the greater curvature is plicated inside the gastric lumen. Our aims were to analyze the effectiveness of gastric plication on the resolution of obesity, impaired glucose tolerance, and fatty liver in an experimental model of diet-induced obesity (DIO) and to evaluate changes in glycerol metabolism, a key substrate for adiposity and gluconeogenesis, in adipose tissue and the liver. METHODS: Male Wistar DIOrats (n = 58) were subjected to surgical (sham operation and gastric plication) or dietary interventions [fed a normal diet (ND) or high-fat diet (HFD) or pair-fed to the amount of food eaten by gastric-plicated animals]. The expression of aquaglyceroporins (AQPs) in epididymal (EWAT) and subcutaneous (SCWAT) fat and the liver was analyzed by real-time PCR and Western blot. RESULTS: Gastric plication did not result in a significant weight loss in DIOrats, showing a modest reduction in whole-body adiposity and hepatic steatosis. However, gastric-plicated animals exhibited an improvement in basal glycemia and glucose clearance, without changes in hepatic gluconeogenic genes. DIO was associated with an increase in glycerol, higher AQP3 and AQP7 in EWAT and SCWAT, and a decrease in hepatic AQP9. Gastric plication downregulated AQP3 in both fat depots without changes in adipose AQP7 and hepatic AQP9. CONCLUSION: Gastric plication results in a modest reduction in adiposity and hepatosteatosis but restores glycemia by downregulating AQP3, which entails lower efflux of glycerol from fat, lower plasma glycerol availability, and a reduced use of glycerol as a substrate for hepatic gluconeogenesis.
Authors: M Martín; M A Burrell; J Gómez-Ambrosi; V Valentí; Á Bueno; B Ramírez; S Becerril; A Lancha; P del Sol Calderón; L Méndez-Giménez; V Catalán; A Rodríguez; S Fernández; M Muñoz-Navas; J A Cienfuegos; G Frühbeck Journal: Obes Surg Date: 2012-04 Impact factor: 4.129
Authors: A Rodríguez; J Gómez-Ambrosi; V Catalán; M J Gil; S Becerril; N Sáinz; C Silva; J Salvador; I Colina; G Frühbeck Journal: Int J Obes (Lond) Date: 2009-02-24 Impact factor: 5.095
Authors: L Méndez-Giménez; S Becerril; S P Camões; I V da Silva; C Rodrigues; R Moncada; V Valentí; V Catalán; J Gómez-Ambrosi; J P Miranda; G Soveral; G Frühbeck; A Rodríguez Journal: Int J Obes (Lond) Date: 2017-06-06 Impact factor: 5.095