Literature DB >> 2805427

The spot-ELISA: a sensitive in vitro method to study the immune response to hepatitis B surface antigen.

P J Wismans1, J van Hattum, G C De Gast, H J Endeman, J Poel, B Stolk, T Maikoe, G C Mudde.   

Abstract

We present our experience with a new sensitive in vitro method for the study of the immune response to hepatitis B surface antigen (HBsAg). This immunoenzymatic technique, called spot-ELISA, detects specific immunoglobulin production (e.g. IgG- or IgM-anti-HBs) by stimulated individual B cells in vitro. We have studied the immune response against HBsAg by mitogenic stimulation and subsequent spot-ELISA assay in 24 well-documented subjects with known immune status after either natural infection or vaccination with plasma-derived vaccine. Results indicate a good correlation between in vitro IgG anti-HBs spots and immune 'memory.' A possible predictive value in non-immunized and non-responder subjects awaits further confirmation.

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Year:  1989        PMID： 2805427      PMCID： PMC1534596     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  38 in total

1.  Is booster injection with hepatitis B vaccine necessary in healthy responders? A study of the immune response.

Authors:  P J Wismans; J van Hattum; G C Mudde; H J Endeman; J Poel; G C de Gast
Journal:  J Hepatol       Date:  1989-03       Impact factor: 25.083

2.  Effect of supplementary vaccination in healthy non-responders to hepatitis B vaccination.

Authors:  P Wismans; J van Hattum; T Stelling; J Poel; G C de Gast
Journal:  Hepatogastroenterology       Date:  1988-04

3.  In vitro anti-HBs antibody synthesis from anti-hepatitis B vaccine recipients.

Authors:  V Barnaba; M Levrero; G Ruberti; A van Dyke; A Perrone; A Musca; F Balsano
Journal:  Clin Exp Immunol       Date:  1987-11       Impact factor: 4.330

4.  Hepatitis B virus-reactive human T lymphocyte clones: antigen specificity and helper function for antibody synthesis.

Authors:  E Celis; P C Kung; T W Chang
Journal:  J Immunol       Date:  1984-03       Impact factor: 5.422

5.  Antibodies recognizing human serum albumin are not elicited by immunization with preS2 sequences of the hepatitis B virus envelope protein.

Authors:  A R Neurath; N Strick; K Parker; S B Kent
Journal:  J Med Virol       Date:  1988-02       Impact factor: 2.327

6.  Antigen-induced production of lymphokines by human T cell clones specific for hepatitis B surface antigen.

Authors:  E Celis; R W Miller; T W Chang
Journal:  Hum Immunol       Date:  1984-12       Impact factor: 2.850

7.  Human tonsil B lymphocyte function. I. The proliferative response to Staphylococcus aureus and pokeweed mitogen in relation to surface heavy chains mu and delta.

Authors:  G C Mudde; C J Verberne; K Groeneveld; G C De Gast
Journal:  Clin Exp Immunol       Date:  1984-06       Impact factor: 4.330

8.  T-lymphocyte subpopulations in patients with various courses after hepatitis B virus infection.

Authors:  J van Hattum; A van Oudenaren; S W Schalm; J W Visser; R Benner
Journal:  Scand J Gastroenterol       Date:  1984-06       Impact factor: 2.423

9.  The immune response against pre-S2-encoded peptides and human serum albumin during hepatitis B virus infection.

Authors:  U B Hellström; S P Sylvan
Journal:  Postgrad Med J       Date:  1987       Impact factor: 2.401

10.  In vitro antigen-induced antibody responses to hepatitis B surface antigen in man. Kinetic and cellular requirements.

Authors:  T R Cupps; J L Gerin; R H Purcell; P K Goldsmith; A S Fauci
Journal:  J Clin Invest       Date:  1984-10       Impact factor: 14.808
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  7 in total

1.  Long-term persistence of T cell memory to HBsAg after hepatitis B vaccination.

Authors:  Ru-Xiang Wang; Greet J Boland; Jan van Hattum; Gijsbert C de Gast
Journal:  World J Gastroenterol       Date:  2004-01-15       Impact factor: 5.742

2.  Can HB vaccine yield a booster effect on individuals with positive serum anti-HBs and anti-HBc markers?

Authors:  Ru-Xiang Wang; Ying Guo; Chang-Hong Yang; Yu Song; Juan Chen; Fu-Sheng Pang; Shao-Ping Lei; Xiao-Ming Jia; Jin-Ying Wen; Christina Y Shi
Journal:  World J Gastroenterol       Date:  2004-01-15       Impact factor: 5.742

3.  Four-year follow up of hepatitis C patients vaccinated against hepatitis B virus.

Authors:  Slawomir Chlabicz; Tadeusz-Wojciech Lapinski; Anna Grzeszczuk; Danuta Prokopowicz
Journal:  World J Gastroenterol       Date:  2005-03-28       Impact factor: 5.742

4.  Strategies for hepatitis B infection.

Authors:  P J Wismans; J van Hattum
Journal:  Gut       Date:  1993-04       Impact factor: 23.059

5.  Secretion of Toxoplasma gondii-specific antibody in vitro by peripheral blood mononuclear cells as a new marker of acute toxoplasmosis.

Authors:  J P Vendrell; F Pratlong; A Decoster; P Boulot; A M Conge; F Darcy; M Segondy; M F Huguet; A Serre
Journal:  Clin Exp Immunol       Date:  1992-07       Impact factor: 4.330

Review 6.  Emerging hepatitis B virus infection in vaccinated populations: a rising concern?

Authors:  Tung-Hung Su; Pei-Jer Chen
Journal:  Emerg Microbes Infect       Date:  2012-09-19       Impact factor: 7.163

Review 7.  Biosensors for Detecting Lymphocytes and Immunoglobulins.

Authors:  Pietro Salvo; Federico M Vivaldi; Andrea Bonini; Denise Biagini; Francesca G Bellagambi; Filippo M Miliani; Fabio Di Francesco; Tommaso Lomonaco
Journal:  Biosensors (Basel)       Date:  2020-10-27
  7 in total

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