| Literature DB >> 28054206 |
Shenqiang Rao1, Shuangmei Liu1, Lifang Zou1, Tianyu Jia1, Shanhong Zhao1, Bing Wu1, Zhihua Yi1, Shouyu Wang1, Yun Xue1, Yun Gao1, Changshui Xu1, Guilin Li1, Hong Xu1, Chunping Zhang1, Shangdong Liang2.
Abstract
Type 2 diabetes mellitus (T2DM) accounts for more than 90% of all cases of diabetes mellitus (DM). Diabetic neuropathic pain (DNP) is a common complication of T2DM. Sinomenine is a natural bioactive component extracted from the Sinomenium acutum and has anti-inflammatory effects. The aim of our study was to investigate the effects of sinomenine on DNP mediated by the P2X3 receptor in dorsal root ganglia (DRG). The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) in T2DM rats were lower than those of control rats. MWT and TWL in T2DM rats treated with sinomenine were higher compared with those in T2DM rats. The expression levels of the P2X3 protein and mRNA in T2DM rat DRG were higher compared with those of the control, while those in T2DM rats treated with sinomenine were significantly lower compared with those of the T2DM rats. Sinomenine significantly inhibited P2X3 agonist ATP-activated currents in HEK293 cells transfected with the P2X3 receptor. Sinomenine decreased the phosphorylation and activation of P38MAPK in T2DM DRG. Therefore, sinomenine treatment may suppress the up-regulated expression and activation of the P2X3 receptor and relieve the hyperalgesia potentiated by the activation of P38MAPK in T2DM rats.Entities:
Keywords: Diabetic neuropathic pain; Dorsal root ganglia; P2X3 receptor; Sinomenine
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Year: 2017 PMID： 28054206 PMCID： PMC5432482 DOI： 10.1007/s11302-016-9554-z
Source DB: PubMed Journal: Purinergic Signal ISSN： 1573-9538 Impact factor: 3.765