Ehsan Alaee1, Behnaz Bazrafshan2, Ali Reza Azaminejad3, Mahnaz Fouladinejad1, Majid Shahbazi4. 1. Assistant Professor, Department of Paediatrics and Neonatology, Neonatal and Children's Health Research Center, Golestan, University of Medical Sciences , Gorgan, Iran . 2. Medical Cellular & Molecular Research Center, Department of Medical Genetics, Taleghani Children's Hospital, Golestan University of Medical Sciences , Gorgan, Iran . 3. Pediatrician, Department of Paediatrics and Neonatology, Golestan University of Medical Sciences , Gorgan, Iran . 4. Medical Cellular & Molecular Research Center, Department of Medical Genetics, Taleghani Children's Hospital, Golestan University of MedicalSciences , Gorgan, Iran .
Abstract
INTRODUCTION: Jaundice is a common condition during the neonatal period. Prolonged jaundice occurs in a large number of breastfed infants. Considering the impact of genetic factors on the incidence of jaundice present study was conducted. AIM: The aim of this study was to determine the association between prolonged jaundice and G71R polymorphism in Gilbert's syndrome. MATERIALS AND METHODS: This case-control study was conducted at Taleghani Children's Hospital of Gorgan, Iran. The study group consisted of 87 icteric patients (aged more than 2 weeks) with an indirect bilirubin level higher than 10mg/dL. The control group consisted of 81 newborns without jaundice. The two groups were matched in terms of age and gender. DNA extraction was performed by "phenol-chloroform" method. Polymerase Chain Reaction with Confronting Two-Pair Primers (PCR-CTPP) was applied to amplify G71R polymorphism. RESULTS: Overall, 84% and 64% of subjects in the study and control groups were male, respectively. The distribution of Gilbert genotype was not significantly different between the two groups (p=0.772). There was a correlation between prolonged jaundice in males and UGT1A1 G71R polymorphism (p =0.03). In the study group, 5(5.7%) subjects were homozygous (for A/A), 73 (83.9%) were heterozygous (for A/G), and 9(10.3%) were normal (for G/G). In the control group, 3(3.7%) participants were homozygous (A/A), 68(84%) were heterozygous (A/G) and 10 (12.3%) were normal (G/G). CONCLUSION: There was no association between prolonged jaundice and G71R polymorphism, even though a relationship was revealed between male gender and the mentioned polymorphism.
INTRODUCTION:Jaundice is a common condition during the neonatal period. Prolonged jaundice occurs in a large number of breastfed infants. Considering the impact of genetic factors on the incidence of jaundice present study was conducted. AIM: The aim of this study was to determine the association between prolonged jaundice and G71R polymorphism in Gilbert's syndrome. MATERIALS AND METHODS: This case-control study was conducted at Taleghani Children's Hospital of Gorgan, Iran. The study group consisted of 87 icteric patients (aged more than 2 weeks) with an indirect bilirubin level higher than 10mg/dL. The control group consisted of 81 newborns without jaundice. The two groups were matched in terms of age and gender. DNA extraction was performed by "phenol-chloroform" method. Polymerase Chain Reaction with Confronting Two-Pair Primers (PCR-CTPP) was applied to amplify G71R polymorphism. RESULTS: Overall, 84% and 64% of subjects in the study and control groups were male, respectively. The distribution of Gilbert genotype was not significantly different between the two groups (p=0.772). There was a correlation between prolonged jaundice in males and UGT1A1G71R polymorphism (p =0.03). In the study group, 5(5.7%) subjects were homozygous (for A/A), 73 (83.9%) were heterozygous (for A/G), and 9(10.3%) were normal (for G/G). In the control group, 3(3.7%) participants were homozygous (A/A), 68(84%) were heterozygous (A/G) and 10 (12.3%) were normal (G/G). CONCLUSION: There was no association between prolonged jaundice and G71R polymorphism, even though a relationship was revealed between male gender and the mentioned polymorphism.
Authors: Libor Vítek; Milan Jirsa; Marie Brodanová; Milan Kalab; Zdenek Marecek; Vilém Danzig; Ladislav Novotný; Petr Kotal Journal: Atherosclerosis Date: 2002-02 Impact factor: 5.162