Literature DB >> 28049781

Isolating DNA from Gram-Negative Bacteria.

Michael R Green, Joseph Sambrook.   

Abstract

The isolation of DNA from bacteria, described in this protocol, relies upon the use of sodium dodecyl sulfate and proteinase K to lyse the cells. High-molecular-weight DNA is then sheared (to reduce its viscosity and make it more manageable), extracted with phenol:chloroform, and precipitated with isopropanol. DNA isolated according to this procedure ranges from 30 to 80 kb in length.
© 2017 Cold Spring Harbor Laboratory Press.

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Year:  2017        PMID： 28049781     DOI： 10.1101/pdb.prot093369

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  5 in total

1.  Optimised biomolecular extraction for metagenomic analysis of microbial biofilms from high-mountain streams.

Authors:  Susheel Bhanu Busi; Paraskevi Pramateftaki; Jade Brandani; Stilianos Fodelianakis; Hannes Peter; Rashi Halder; Paul Wilmes; Tom J Battin
Journal:  PeerJ       Date:  2020-10-27       Impact factor: 2.984

2.  Assessment of In-Vitro Synergy of Fosfomycin with Meropenem, Amikacin and Tigecycline in Whole Genome Sequenced Extended and Pan Drug Resistant Klebsiella Pneumoniae: Exploring A Colistin Sparing Protocol.

Authors:  Manawr Al-Quraini; Meher Rizvi; Zaaima Al-Jabri; Hiba Sami; Muna Al-Muzahmi; Zakariya Al-Muharrmi; Neelam Taneja; Ibrahim Al-Busaidi; Rajeev Soman
Journal:  Antibiotics (Basel)       Date:  2022-01-25

3.  Genomic and Phenotypic Characterization of a Colistin-Resistant Escherichia coli Isolate Co-Harboring bla NDM-5, bla OXA-1, and bla CTX-M-55 Isolated from Urine.

Authors:  Jingchen Hao; Zhangrui Zeng; Xue Xiao; Yinhuan Ding; Jiamin Deng; Yueshuai Wei; Jinbo Liu
Journal:  Infect Drug Resist       Date:  2022-03-29       Impact factor: 4.003

4.  Thermus thermophilus Argonaute Functions in the Completion of DNA Replication.

Authors:  Samson M Jolly; Ildar Gainetdinov; Karina Jouravleva; Han Zhang; Lara Strittmatter; Shannon M Bailey; Gregory M Hendricks; Avantika Dhabaria; Beatrix Ueberheide; Phillip D Zamore
Journal:  Cell       Date:  2020-08-25       Impact factor: 41.582

5.  Emergence of NDM-5-Producing Escherichia coli in a Teaching Hospital in Chongqing, China: IncF-Type Plasmids May Contribute to the Prevalence of bla NDM- 5.

Authors:  Hua Zou; Xiaojiong Jia; Hang Liu; Shuang Li; Xianan Wu; Shifeng Huang
Journal:  Front Microbiol       Date:  2020-03-06       Impact factor: 5.640
  5 in total

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