Literature DB >> 28044150

A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy.

Gabriela Matela1, Peng Gao2, Gernot Guigas1, Antonia F Eckert1, Karin Nienhaus1, G U Nienhaus3.   

Abstract

Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

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Year:  2017        PMID： 28044150     DOI： 10.1039/c6cc09081h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  11 in total

1.  Monomerization of far-red fluorescent proteins.

Authors:  Timothy M Wannier; Sarah K Gillespie; Nicholas Hutchins; R Scott McIsaac; Sheng-Yi Wu; Yi Shen; Robert E Campbell; Kevin S Brown; Stephen L Mayo
Journal:  Proc Natl Acad Sci U S A       Date:  2018-11-13       Impact factor: 11.205

2.  Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter.

Authors:  Premashis Manna; Sheng-Ting Hung; Srijit Mukherjee; Pia Friis; David M Simpson; Maria N Lo; Amy E Palmer; Ralph Jimenez
Journal:  Integr Biol (Camb)       Date:  2018-09-17       Impact factor: 2.192

3.  STED super-resolved microscopy.

Authors:  Giuseppe Vicidomini; Paolo Bianchini; Alberto Diaspro
Journal:  Nat Methods       Date:  2018-01-29       Impact factor: 28.547

4.  A guide to choosing fluorescent protein combinations for flow cytometric analysis based on spectral overlap.

Authors:  Benjamin Kleeman; Andre Olsson; Tess Newkold; Matt Kofron; Monica DeLay; David Hildeman; H Leighton Grimes
Journal:  Cytometry A       Date:  2018-03-13       Impact factor: 4.355

Review 5.  AIEgen-Based Fluorescent Nanomaterials: Fabrication and Biological Applications.

Authors:  Hui Gao; Xin Zhao; Sijie Chen
Journal:  Molecules       Date:  2018-02-14       Impact factor: 4.411

6.  Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.

Authors:  M Bernhardt; J-D Nicolas; M Osterhoff; H Mittelstädt; M Reuss; B Harke; A Wittmeier; M Sprung; S Köster; T Salditt
Journal:  Nat Commun       Date:  2018-09-07       Impact factor: 14.919

7.  Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

Authors:  Maria Kamper; Haisen Ta; Nickels A Jensen; Stefan W Hell; Stefan Jakobs
Journal:  Nat Commun       Date:  2018-11-12       Impact factor: 14.919

8.  Bright GFP with subnanosecond fluorescence lifetime.

Authors:  Anastasia V Mamontova; Ilya D Solovyev; Alexander P Savitsky; Alexander М Shakhov; Konstantin A Lukyanov; Alexey M Bogdanov
Journal:  Sci Rep       Date:  2018-09-05       Impact factor: 4.379

9.  Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins.

Authors:  Laura Canty; Santosh Hariharan; Qian Liu; Steven A Haney; David W Andrews
Journal:  PLoS One       Date:  2018-11-28       Impact factor: 3.240

10.  Deciphering the Role of Positions 145 and 165 in Fluorescence Lifetime Shortening in the EGFP Variants.

Authors:  Anastasia V Mamontova; Aleksander M Shakhov; Konstantin A Lukyanov; Alexey M Bogdanov
Journal:  Biomolecules       Date:  2020-11-13
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