Vincent Hivernaud1, Bruno Lefourn2, Myriam Robard3, Jérôme Guicheux4, Pierre Weiss4. 1. INSERM, LIOAD, UMR-S 791, Nantes, France; Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, France; ONIRIS, UMR-S 791, LIOAD, Nantes, France. Electronic address: vincent.hivernaud@hotmail.fr. 2. Clinique Bretéché, Département de Chirurgie Plastique et Reconstructive, Nantes, France. 3. Cellular and Tissular Imaging Core Facility of Nantes University (MicroPICell), Structure Fédérative de Recherche François Bonamy, Université de Nantes, Nantes, France. 4. INSERM, LIOAD, UMR-S 791, Nantes, France; Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, France; ONIRIS, UMR-S 791, LIOAD, Nantes, France; CHU Nantes, PHU 4 OTONN, Nantes, France.
Abstract
BACKGROUND: Autologous fat grafting is a widely used technique that gives natural results when treating soft tissue deficiencies. However, there is no consensus on which is the best procedure to use, leading to unpredictable results because of fat graft resorption. OBJECTIVES: This study compared four commercial lipotransfer devices by analyzing the behavior of the processed adipose tissue and outcome of the adipose graft in an in vivo model. METHODS: Four different protocols that used manual, power-assisted or water-assisted lipoaspiration and then decantation, centrifugation, or filtration were used on each of eight patients to process lipoaspirate. Harvested adipose tissue was assessed in vitro for tissue resorption, oil formation, and cytokine secretion. Graft resorption rate was calculated and histological analyses were performed after subcutaneously injecting the harvested adipose tissue in a murine model. RESULTS: All protocols resulted in very low oil formation and histologically healthy grafts. The tissue volume was significantly greater after 2 days in culture when using manual lipoaspiration and soft centrifugations/washing steps (Microfill®/Macrofill®) compared to Water-Assisted Lipoaspiration/Decantation (BodyJet®) and Power-Assisted Lipoaspiration/Filtration (PAL® + PureGraft®). These results were confirmed in mice 1 month after subcutaneous injection, with greater efficiency obtained with protocols that used (A) manual aspiration, (B) soft centrifugations, and (C) washing steps. CONCLUSIONS: We confirmed that the choice of technique used to process adipose tissue during lipotransfer surgery can highly influence fat grafting efficacy. In our study, the use of manual aspiration combined with soft centrifugations led to the best results in the selected models.
BACKGROUND: Autologous fat grafting is a widely used technique that gives natural results when treating soft tissue deficiencies. However, there is no consensus on which is the best procedure to use, leading to unpredictable results because of fat graft resorption. OBJECTIVES: This study compared four commercial lipotransfer devices by analyzing the behavior of the processed adipose tissue and outcome of the adipose graft in an in vivo model. METHODS: Four different protocols that used manual, power-assisted or water-assisted lipoaspiration and then decantation, centrifugation, or filtration were used on each of eight patients to process lipoaspirate. Harvested adipose tissue was assessed in vitro for tissue resorption, oil formation, and cytokine secretion. Graft resorption rate was calculated and histological analyses were performed after subcutaneously injecting the harvested adipose tissue in a murine model. RESULTS: All protocols resulted in very low oil formation and histologically healthy grafts. The tissue volume was significantly greater after 2 days in culture when using manual lipoaspiration and soft centrifugations/washing steps (Microfill®/Macrofill®) compared to Water-Assisted Lipoaspiration/Decantation (BodyJet®) and Power-Assisted Lipoaspiration/Filtration (PAL® + PureGraft®). These results were confirmed in mice 1 month after subcutaneous injection, with greater efficiency obtained with protocols that used (A) manual aspiration, (B) soft centrifugations, and (C) washing steps. CONCLUSIONS: We confirmed that the choice of technique used to process adipose tissue during lipotransfer surgery can highly influence fat grafting efficacy. In our study, the use of manual aspiration combined with soft centrifugations led to the best results in the selected models.
Authors: Jan-Willem Groen; Todor K Krastev; Juliette Hommes; Janneke A Wilschut; Marco J P F Ritt; Rene R J W van der Hulst Journal: Plast Reconstr Surg Glob Open Date: 2017-12-22