Lukas Fasbender1, Daniel Maurer2, Jürgen Kreuzwieser2, Ines Kreuzer3, Waltraud X Schulze4, Jörg Kruse2, Dirk Becker3, Saleh Alfarraj5, Rainer Hedrich3,5, Christiane Werner1, Heinz Rennenberg2,5. 1. Institute of Forest Sciences, Chair of Ecosystem Physiology, University of Freiburg, Georges-Köhler-Allee 53/54, Freiburg, 79110, Germany. 2. Institute of Forest Sciences, Chair of Tree Physiology, University of Freiburg, Georges-Köhler-Allee 53/54, Freiburg, 79110, Germany. 3. Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Würzburg, 97070, Germany. 4. Department of Plant Systems Biology, University of Hohenheim, Stuttgart, 70593, Germany. 5. College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.
Abstract
The present study was performed to elucidate the fate of carbon (C) and nitrogen (N) derived from protein of prey caught by carnivorous Dionaea muscipula. For this, traps were fed 13 C/15 N-glutamine (Gln). The release of 13 CO2 was continuously monitored by isotope ratio infrared spectrometry. After 46 h, the allocation of C and N label into different organs was determined and tissues were subjected to metabolome, proteome and transcriptome analyses. Nitrogen of Gln fed was already separated from its C skeleton in the decomposing fluid secreted by the traps. Most of the Gln-C and Gln-N recovered inside plants were localized in fed traps. Among nonfed organs, traps were a stronger sink for Gln-C compared to Gln-N, and roots were a stronger sink for Gln-N compared to Gln-C. A significant amount of the Gln-C was respired as indicated by 13 C-CO2 emission, enhanced levels of metabolites of respiratory Gln degradation and increased abundance of proteins of respiratory processes. Transcription analyses revealed constitutive expression of enzymes involved in Gln metabolism in traps. It appears that prey not only provides building blocks of cellular constituents of carnivorous Dionaea muscipula, but also is used for energy generation by respiratory amino acid degradation.
The present study was performed to elucidate the fate of carbon (C) and n class="Chemical">nitrogen (N) derived from protein of prey caught by carnivorous Dionaea muscipula. For this, traps were fed 13 C/15 N-glutamine (Gln). The release of 13 CO2 was continuously monitored by isotope ratio infrared spectrometry. After 46 h, the allocation of C and N label into different organs was determined and tissues were subjected to metabolome, proteome and transcriptome analyses. Nitrogen of Gln fed was already separated from its C skeleton in the decomposing fluid secreted by the traps. Most of the Gln-C and Gln-N recovered inside plants were localized in fed traps. Among nonfed organs, traps were a stronger sink for Gln-C compared to Gln-N, and roots were a stronger sink for Gln-N compared to Gln-C. A significant amount of the Gln-C was respired as indicated by 13 C-CO2 emission, enhanced levels of metabolites of respiratory Gln degradation and increased abundance of proteins of respiratory processes. Transcription analyses revealed constitutive expression of enzymes involved in Gln metabolism in traps. It appears that prey not only provides building blocks of cellular constituents of carnivorous Dionaea muscipula, but also is used for energy generation by respiratory amino acid degradation.
Authors: Daniel Maurer; Daniel Weber; Eva Ballering; Salah Alfarraj; Gada Albasher; Rainer Hedrich; Christiane Werner; Heinz Rennenberg Journal: Ann Bot Date: 2020-03-09 Impact factor: 4.357
Authors: Renate Sachse; Anna Westermeier; Max Mylo; Joey Nadasdi; Manfred Bischoff; Thomas Speck; Simon Poppinga Journal: Proc Natl Acad Sci U S A Date: 2020-06-22 Impact factor: 11.205
Authors: Paul G Nevill; Katharine A Howell; Adam T Cross; Anna V Williams; Xiao Zhong; Julian Tonti-Filippini; Laura M Boykin; Kingsley W Dixon; Ian Small Journal: Genome Biol Evol Date: 2019-02-01 Impact factor: 3.416