Olivier Abbo1, Marion Taurand2, Paul Monsarrat3, Isabelle Raymond4, Emmanuelle Arnaud2, Sandra De Barros2, Françoise Auriol5, Philippe Galinier5, Louis Casteilla2, Valerie Planat-Benard6. 1. STROMALab, Université de Toulouse, Toulouse, France; Service de Chirurgie Pédiatrique, Hôpital des Enfants de Toulouse, Toulouse, France. 2. STROMALab, Université de Toulouse, Toulouse, France. 3. STROMALab, Université de Toulouse, Toulouse, France; Toulouse Faculty of Dentistry, Paul Sabatier University (UPS) and Toulouse University Hospital, Toulouse, France. 4. STROMALab, Université de Toulouse, Toulouse, France; University Paul Sabatier of Toulouse (UPS), Institut National Polytechnique - Ecole Nationale Vétérinaire de Toulouse (INP-ENVT), Plateforme d'Histopathologie Expérimentale et Comparée, Toulouse, France. 5. Toulouse Faculty of Dentistry, Paul Sabatier University (UPS) and Toulouse University Hospital, Toulouse, France. 6. STROMALab, Université de Toulouse, Toulouse, France. Electronic address: valerie.planat@inserm.fr.
Abstract
BACKGROUND: Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications. METHODS: Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia. RESULTS: Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties. CONCLUSION: This is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.
BACKGROUND: Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications. METHODS: Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia. RESULTS: Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties. CONCLUSION: This is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.
Authors: Natalie N Chung; Ryan C Ransom; Charles P Blackshear; Dre M Irizarry; Derek Yen; Arash Momeni; Gordon K Lee; Dung H Nguyen; Michael T Longaker; Derrick C Wan Journal: Plast Reconstr Surg Date: 2019-04 Impact factor: 4.730
Authors: Andrea L DiCarlo; Radia Tamarat; Carmen I Rios; Marc Benderitter; Christine W Czarniecki; Theresa C Allio; Francesca Macchiarini; Bert W Maidment; Jean-Rene Jourdain Journal: Radiat Res Date: 2017-06-12 Impact factor: 2.841