| Literature DB >> 28040164 |
Juliana de Lima Marques1, Graciele Daiana Funck2, Guilherme da Silva Dannenberg2, Claudio Eduardo Dos Santos Cruxen2, Shanise Lisie Mello El Halal2, Alvaro Renato Guerra Dias2, Ângela Maria Fiorentini2, Wladimir Padilha da Silva3.
Abstract
The aim of this study was to evaluate the effectiveness of a biodegradable film, with antimicrobial metabolites produced by Lactobacillus curvatus P99 incorporated, targeting the control of Listeria monocytogenes in sliced "Prato" cheese. Tests were performed to evaluate the spectrum of action of cell-free supernatant (CFS) of P99 against different microorganisms, as well as to detect the minimum inhibitory (MIC) and bactericidal (MBC) concentrations against L. monocytogenes Scott A. The detection of genes that encode for the production of bacteriocins and evaluation of their expression were performed. Antimicrobial films were prepared, followed by in vitro and in situ analysis. The MIC and MBC of CFS against L. monocytogenes Scott A was 15.6 μL/mL and 62.5 μL/mL, respectively. Lactobacillus curvatus P99 presented two genes coding for the bacteriocins, which were expressed. Films with added MBC showed activity against different indicator microorganisms and were able to control L. monocytogenes Scott A when used in sliced "Prato" cheese.Entities:
Keywords: Antimicrobial film; Bacteriocin; Biopreservative; Sliced cheese
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Year: 2016 PMID： 28040164 DOI： 10.1016/j.fm.2016.11.008
Source DB: PubMed Journal: Food Microbiol ISSN： 0740-0020 Impact factor: 5.516