| Literature DB >> 28039799 |
Ignacio Fernandez-Sainz1, Gisselle N Medina2, Elizabeth Ramirez-Medina3, Marla J Koster4, Marvin J Grubman4, Teresa de Los Santos5.
Abstract
A human adenovirus (Ad5) vectored foot-and-mouth disease virus (FMDV) O1-Manisa subunit vaccine (Ad5-O1Man) was engineered to deliver FMDV O1-Manisa capsid and capsid-processing proteins. Swine inoculated with Ad5-O1Man developed an FMDV-specific humoral response as compared to animals inoculated with an empty Ad5-vector. Vaccinated animals were completely protected against homologous challenge at 7 or 21 days post-vaccination. Potency studies exhibited a PD50 of about 107 pfu/animal while a dose of 4×107pfu/animal fully protected swine against FMDV intradermal challenge. In-vitro cross-neutralization analysis distinctly predicted that swine vaccinated with Ad5-O1Man would be protected against challenge with homologous FMDV O1Man Middle East-South Asia (ME-SA) topotype and also against recent outbreak strains of Mya-98 South East Asia (SEA) lineage including O1-UK-2001 and O1-South Korea-2010. These results indicate that recombinant Ad5-O1Man is an effective, safe and cross-reacting vaccine that could potentially be used preventively and in outbreak situations, to control FMDV O Mya-98 lineage in swine. Published by Elsevier Inc.Entities:
Keywords: Ad5-FMD; Adenovirus; Cross-protection; Disease; FMDV; Foot-and-mouth; O1Manisa; Swine; Vaccine
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Year: 2016 PMID： 28039799 DOI： 10.1016/j.virol.2016.12.021
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616