Saadettin Sel1, Eva Patzel2, Lucia Poggi2, Delia Kaiser2, Thomas Kalinski3, Martin Schicht4, Friedrich Paulsen4, Norbert Nass3. 1. Department of Ophthalmology, University Heidelberg, Germany. Electronic address: saadettinsel@googlemail.com. 2. Department of Ophthalmology, University Heidelberg, Germany. 3. Institute of Pathology, University Magdeburg, Germany. 4. Institut for Anatomy II, University Erlangen, Germany.
Abstract
BACKGROUND: Neuronatin (Nnat) was initially identified as a highly expressed gene in neonatal mammalian brain. In this study, we analyze the spatial and temporal expression pattern of Nnat during mouse eye development as well as in the adult. METHODS: The expression of Nnat was analyzed on mRNA as well as protein level. The presence of Nnat transcripts in the adult retina was examined using reverse transcription-polymerase chain reaction (RT-PCR). Nnat protein expression was evaluated by Western blot and immunohistochemistry during eye development at embryonic day (E) 12, 15, 16 and postnatal day (P) 7, 14, 30 and 175 (adult). RESULTS: Immunohistochemical studies of the developing mouse eye revealed Nnat expression in embryonic and adult neuroretina as well as in corneal epithelial, stromal, endothelial cells and in lens epithelium. Expression of Nnat was detected from E12 onwards and was also present in adult eyes. CONCLUSIONS: The expression pattern suggests that Nnat may play an important role during eye development and in the maintenance of mature eye.
BACKGROUND:Neuronatin (Nnat) was initially identified as a highly expressed gene in neonatal mammalian brain. In this study, we analyze the spatial and temporal expression pattern of Nnat during mouse eye development as well as in the adult. METHODS: The expression of Nnat was analyzed on mRNA as well as protein level. The presence of Nnat transcripts in the adult retina was examined using reverse transcription-polymerase chain reaction (RT-PCR). Nnat protein expression was evaluated by Western blot and immunohistochemistry during eye development at embryonic day (E) 12, 15, 16 and postnatal day (P) 7, 14, 30 and 175 (adult). RESULTS: Immunohistochemical studies of the developing mouse eye revealed Nnat expression in embryonic and adult neuroretina as well as in corneal epithelial, stromal, endothelial cells and in lens epithelium. Expression of Nnat was detected from E12 onwards and was also present in adult eyes. CONCLUSIONS: The expression pattern suggests that Nnat may play an important role during eye development and in the maintenance of mature eye.
Authors: Elena Perenthaler; Anita Nikoncuk; Soheil Yousefi; Woutje M Berdowski; Maysoon Alsagob; Ivan Capo; Herma C van der Linde; Paul van den Berg; Edwin H Jacobs; Darija Putar; Mehrnaz Ghazvini; Eleonora Aronica; Wilfred F J van IJcken; Walter G de Valk; Evita Medici-van den Herik; Marjon van Slegtenhorst; Lauren Brick; Mariya Kozenko; Jennefer N Kohler; Jonathan A Bernstein; Kristin G Monaghan; Amber Begtrup; Rebecca Torene; Amna Al Futaisi; Fathiya Al Murshedi; Renjith Mani; Faisal Al Azri; Erik-Jan Kamsteeg; Majid Mojarrad; Atieh Eslahi; Zaynab Khazaei; Fateme Massinaei Darmiyan; Mohammad Doosti; Ehsan Ghayoor Karimiani; Jana Vandrovcova; Faisal Zafar; Nuzhat Rana; Krishna K Kandaswamy; Jozef Hertecant; Peter Bauer; Mohammed A AlMuhaizea; Mustafa A Salih; Mazhor Aldosary; Rawan Almass; Laila Al-Quait; Wafa Qubbaj; Serdar Coskun; Khaled O Alahmadi; Muddathir H A Hamad; Salem Alwadaee; Khalid Awartani; Anas M Dababo; Futwan Almohanna; Dilek Colak; Mohammadreza Dehghani; Mohammad Yahya Vahidi Mehrjardi; Murat Gunel; A Gulhan Ercan-Sencicek; Gouri Rao Passi; Huma Arshad Cheema; Stephanie Efthymiou; Henry Houlden; Aida M Bertoli-Avella; Alice S Brooks; Kyle Retterer; Reza Maroofian; Namik Kaya; Tjakko J van Ham; Tahsin Stefan Barakat Journal: Acta Neuropathol Date: 2019-12-09 Impact factor: 17.088