Peibin Zeng1, Zhendong Yang2, Sonia Bakkour3, Bingjun Wang2, Shuli Qing2, Jingxing Wang4, Limin Chen2, Michael Busch3, Hua Shan5, Jing Liu6, Tzong-Hae Lee4. 1. West China School of Public Health, Sichuan University, Chengdu, Sichuan, China. 2. Xinyang 154 Military Hospital, Xinyang, China. 3. Blood Systems Research Institute, San Francisco, California. 4. Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, Sichuan, China. 5. Department of Pathology, Stanford University, Palo Alto, California. 6. Department of Pathology, Johns Hopkins University, Baltimore, Maryland.
Abstract
BACKGROUND: Severe fever with thrombocytopenia syndrome bunyavirus (sftsv) is an emerging tick-borne rna virus recently identified as the pathogen that causes severe fever with thrombocytopenia syndrome (sfts) in china. the existing commercial nucleic acid testing (comnat) assay with a relatively high claimed limit of quantitative detection (loqd) is not capable of sensitive detection and quantitation of sftsv. Thus, a new real-time reverse transcriptase (rt)-pcr assay with improved sensitivity is needed for clinical diagnosis; it could also be used to screen blood donors if necessary. MATERIALS AND METHODS: We developed a new sftsv rt-pcr nat assay (newnat). About 129 plasma samples from 93 suspected sfts patients with typical clinical symptoms were tested using an anti-sftsv total antibody elisa and both comnat and newnat. The test performance of the two nat assays was evaluated and compared. RESULTS: The newnat had a lower limit for quantitative testing compared to comnat. Twelve samples were comnat negative but newnat positive. Out of 35 suspected sfts patients who were comnat negative and anti-sftsv total antibody negative, four tested positive by the newnat assay and one of these four seroconverted within 2-4 days after testing newnat positive. A high correlation was observed between the cts of the newnat and comnat assays. CONCLUSION: The newnat assay was sensitive for quantitative detection of sftsv and may be applicable to clinical diagnosis and studies of the need for blood donor screening.
BACKGROUND:Severe fever with thrombocytopenia syndrome bunyavirus (sftsv) is an emerging tick-borne rna virus recently identified as the pathogen that causes severe fever with thrombocytopenia syndrome (sfts) in china. the existing commercial nucleic acid testing (comnat) assay with a relatively high claimed limit of quantitative detection (loqd) is not capable of sensitive detection and quantitation of sftsv. Thus, a new real-time reverse transcriptase (rt)-pcr assay with improved sensitivity is needed for clinical diagnosis; it could also be used to screen blood donors if necessary. MATERIALS AND METHODS: We developed a new sftsv rt-pcr nat assay (newnat). About 129 plasma samples from 93 suspected sfts patients with typical clinical symptoms were tested using an anti-sftsv total antibody elisa and both comnat and newnat. The test performance of the two nat assays was evaluated and compared. RESULTS: The newnat had a lower limit for quantitative testing compared to comnat. Twelve samples were comnat negative but newnat positive. Out of 35 suspected sfts patients who were comnat negative and anti-sftsv total antibody negative, four tested positive by the newnat assay and one of these four seroconverted within 2-4 days after testing newnat positive. A high correlation was observed between the cts of the newnat and comnat assays. CONCLUSION: The newnat assay was sensitive for quantitative detection of sftsv and may be applicable to clinical diagnosis and studies of the need for blood donor screening.
Authors: Evan M Bloch; Tzong-Hae Lee; Peter J Krause; Sam R Telford; Lani Montalvo; Daniel Chafets; Sahar Usmani-Brown; Timothy J Lepore; Michael P Busch Journal: Transfusion Date: 2013-01-30 Impact factor: 3.157
Authors: Harry M Savage; Marvin S Godsey; Amy Lambert; Nicholas A Panella; Kristen L Burkhalter; Jessica R Harmon; R Ryan Lash; David C Ashley; William L Nicholson Journal: Am J Trop Med Hyg Date: 2013-07-22 Impact factor: 2.345