Sandeep Kumar1, Gaurav Bhanjana1, Ritesh Kumar Verma1, Dinesh Dhingra2, Neeraj Dilbaghi1, Ki-Hyun Kim3. 1. Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India. 2. Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India. 3. Department of Civil & Environmental Engineering, Hanyang University, Seoul, 04763, Korea.
Abstract
OBJECTIVES: Present modalities for the diagnosis and treatment of diabetes still suffer from certain limitations such as erratic absorption, need of high dose, poor sensitivity or specificity, resistance, substantial morbidity and mortality, long-term complications, and patient-to-patient variability with lifetime treatment. METHODS: This study focused on the development of a water-in-oil-in-water metformin nanoemulsion as an effective method in diabetes treatment. As a Biopharmaceutics Classification System (BCS) class III drug, metformin is hydrophilic in nature with high solubility and poor absorption characteristics. To simultaneously facilitate gastrointestinal absorption and intestinal permeability, metformin was loaded into alginate nanocapsules prepared by an emulsion cross-linking technology. KEY FINDINGS: These prepared metformin-loaded alginate nanoparticles (MLANs) were characterized using transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and photon correlation spectroscopy (PCS)-based particle size analysis. CONCLUSIONS: The drug loading and encapsulation efficiency in MLANs were 3.12 mg (the amount of metformin added in 100 mg of nanoparticles) and 78%, respectively. The results of in-vitro drug release studies and in-vivo efficacy tests (using animal models) demonstrated enhanced efficiency and response of MLANs relative to pure metformin. The efficacy of MLANs (46.8 mg/kg) was overall about three times higher than that of pure metformin150 mg/kg.
OBJECTIVES: Present modalities for the diagnosis and treatment of diabetes still suffer from certain limitations such as erratic absorption, need of high dose, poor sensitivity or specificity, resistance, substantial morbidity and mortality, long-term complications, and patient-to-patient variability with lifetime treatment. METHODS: This study focused on the development of a water-in-oil-in-watermetformin nanoemulsion as an effective method in diabetes treatment. As a Biopharmaceutics Classification System (BCS) class III drug, metformin is hydrophilic in nature with high solubility and poor absorption characteristics. To simultaneously facilitate gastrointestinal absorption and intestinal permeability, metformin was loaded into alginate nanocapsules prepared by an emulsion cross-linking technology. KEY FINDINGS: These prepared metformin-loaded alginate nanoparticles (MLANs) were characterized using transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and photon correlation spectroscopy (PCS)-based particle size analysis. CONCLUSIONS: The drug loading and encapsulation efficiency in MLANs were 3.12 mg (the amount of metformin added in 100 mg of nanoparticles) and 78%, respectively. The results of in-vitro drug release studies and in-vivo efficacy tests (using animal models) demonstrated enhanced efficiency and response of MLANs relative to pure metformin. The efficacy of MLANs (46.8 mg/kg) was overall about three times higher than that of pure metformin150 mg/kg.
Authors: Aline de Sousa Barbosa Freitas Pereira; Maria Laura de Souza Lima; Arnobio Antonio da Silva-Junior; Emanuell Dos Santos Silva; Raimundo Fernandes de Araújo Júnior; Agnes Andrade Martins; Jovelina Samara Ferreira Alves; Artur de Santana Oliveira; Leandro De Santis Ferreira; Emily Cintia Tossi de Araújo Costa; Gerlane Coelho Bernardo Guerra; Caroline Addison Carvalho Xavier de Medeiros; Gerly A C Brito; Renata Ferreira de Carvalho Leitao; Aurigena Antunes de Araújo Journal: Pharm Biol Date: 2021-12 Impact factor: 3.503
Authors: Héctor Hernández-Parra; Hernán Cortés; José Arturo Avalos-Fuentes; María Del Prado-Audelo; Benjamín Florán; Gerardo Leyva-Gómez; Javad Sharifi-Rad; William C Cho Journal: J Nanobiotechnology Date: 2022-09-15 Impact factor: 9.429