| Literature DB >> 28031533 |
Ekaterina A Potter1, Evgenia V Dolgova1, Anastasia S Proskurina1, Yaroslav R Efremov1,2, Alexandra M Minkevich1, Aleksey S Rozanov1, Sergey E Peltek1, Valeriy P Nikolin1, Nelly A Popova1,2, Igor A Seledtsov3, Vladimir V Molodtsov2,3, Evgeniy L Zavyalov1, Oleg S Taranov4, Sergey I Baiborodin1, Alexander A Ostanin5, Elena R Chernykh5, Nikolay A Kolchanov1, Sergey S Bogachev1.
Abstract
Using the ability of poorly differentiated cells to natively internalize fragments of extracellular double-stranded DNA as a marker, we isolated a tumorigenic subpopulation present in Krebs-2 ascites that demonstrated the features of tumor-inducing cancer stem cells. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, we identified a set of 168 genes specifically expressed in TAMRA-positive cells (tumor-initiating stem cells), these genes remaining silent in TAMRA-negative cancer cells. TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells. The observed expression differences between TAMRA+ and TAMRA- cells were validated by Real Time PCR. The results obtained corroborated the biological data that TAMRA+ murine Krebs-2 tumor cells are tumor-initiating stem cells. The approach developed can be applied to profile any poorly differentiated cell types that are capable of immanent internalization of double-stranded DNA.Entities:
Keywords: DNA internalization; RNAseq; Real Time PCR; TAMRA; tumor-initiating stem cells
Mesh：
Substances：
Year: 2017 PMID： 28031533 PMCID： PMC5354742 DOI： 10.18632/oncotarget.14116
Source DB: PubMed Journal: Oncotarget ISSN： 1949-2553