Sebastiaan J van Hal1,2, Björn A Espedido1,3, Geoffrey W Coombs4,5, Benjamin P Howden6,7, Tony M Korman8, Graeme R Nimmo9, Iain B Gosbell1,3,10, Slade O Jensen1,3. 1. School of Medicine, Western Sydney University, Sydney, NSW, Australia. 2. Royal Prince Alfred Hospital, Sydney, NSW, Australia. 3. Antimicrobial Resistance and Mobile Elements Group, Ingham Institute for Applied Medical Research, Sydney, NSW, Australia. 4. School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia. 5. PathWest Laboratory Medicine, Fiona Stanley Hospital, Perth, WA, Australia. 6. Austin Health, Melbourne, Vic., Australia. 7. Department of Microbiology and Immunology, The University of Melbourne, Melbourne, Vic., Australia. 8. Monash Hospital, Melbourne, Vic., Australia. 9. Pathology Queensland, Brisbane, QLD, Australia. 10. Sydney South Western Pathology Service, NSW Pathology, Sydney, NSW, Australia.
Abstract
Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA -positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn 1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associated with the dissemination of a single 'dominant' ST or vanA -encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.
Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA -positive Enterococcus faeciumpatient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn 1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associated with the dissemination of a single 'dominant' ST or vanA -encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.
Authors: Lindsay A Rogers; Kayla Strong; Susan C Cork; Tim A McAllister; Karen Liljebjelke; Rahat Zaheer; Sylvia L Checkley Journal: Front Public Health Date: 2021-06-10
Authors: Xuewei Zhou; Rob J L Willems; Alexander W Friedrich; John W A Rossen; Erik Bathoorn Journal: Antimicrob Resist Infect Control Date: 2020-08-10 Impact factor: 4.887