Izabela Laczmanska1, Pawel Skiba2, Pawel Karpinski2, Marek Bebenek3, Maria M Sasiadek2. 1. Genetics Department, Wroclaw Medical University, Wroclaw, Poland iza.laczmanska@gmail.com. 2. Genetics Department, Wroclaw Medical University, Wroclaw, Poland. 3. 1st Department of Surgical Oncology, Lower Silesian Oncology Center, Wroclaw, Poland.
Abstract
BACKGROUND/AIM: Molecular mechanisms of alterations in protein tyrosine phosphatases (PTPs) genes in cancer have been previously described and include chromosomal aberrations, gene mutations, and epigenetic silencing. However, little is known about small intragenic gains and losses that may lead to either changes in expression or enzyme activity and even loss of protein function. MATERIALS AND METHODS: The aim of this study was to investigate 25 phosphatase genes using customized array comparative genomic hybridization in 16 sporadic colorectal cancer tissues. RESULTS: The analysis revealed two unique small alterations: of 2 kb in PTPN14 intron 1 and of 1 kb in PTPRJ intron 1. We also found gains and losses of whole PTPs gene sequences covered by large chromosome aberrations. CONCLUSION: In our preliminary studies using high-resolution custom microarray we confirmed that PTPs are frequently subjected to whole-gene rearrangements in colorectal cancer, and we revealed that non-polymorphic intragenic changes are rare. Copyright
BACKGROUND/AIM: Molecular mechanisms of alterations in protein tyrosine phosphatases (PTPs) genes in cancer have been previously described and include chromosomal aberrations, gene mutations, and epigenetic silencing. However, little is known about small intragenic gains and losses that may lead to either changes in expression or enzyme activity and even loss of protein function. MATERIALS AND METHODS: The aim of this study was to investigate 25 phosphatase genes using customized array comparative genomic hybridization in 16 sporadic colorectal cancer tissues. RESULTS: The analysis revealed two unique small alterations: of 2 kb in PTPN14 intron 1 and of 1 kb in PTPRJ intron 1. We also found gains and losses of whole PTPs gene sequences covered by large chromosome aberrations. CONCLUSION: In our preliminary studies using high-resolution custom microarray we confirmed that PTPs are frequently subjected to whole-gene rearrangements in colorectal cancer, and we revealed that non-polymorphic intragenic changes are rare. Copyright
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Authors: Claudia A L Ruivenkamp; Tom van Wezel; Carlo Zanon; Alphons P M Stassen; Cestmir Vlcek; Tamás Csikós; Anita M Klous; Nikos Tripodis; Anastassis Perrakis; Lucie Boerrigter; Peter C Groot; Jan Lindeman; Wolter J Mooi; Gerrit A Meijjer; Gert Scholten; Hans Dauwerse; Vaclav Paces; Nico van Zandwijk; Gert Jan B van Ommen; Peter Demant Journal: Nat Genet Date: 2002-06-24 Impact factor: 38.330
Authors: Joanna Kozlowska; Pawel Karpinski; Elzbieta Szmida; Izabela Laczmanska; Blazej Misiak; David Ramsey; Marek Bebenek; Wojciech Kielan; Karolina A Pesz; Maria M Sasiadek Journal: Tumour Biol Date: 2012-01-25