Danijela M Cvetković1, Marko N Živanović2, Milena G Milutinović3, Tijana R Djukić4, Miloš D Radović5, Aleksandar M Cvetković6, Nenad D Filipović7, Nebojša D Zdravković8. 1. University of Kragujevac, Faculty of Science, Institute of Biology and Ecology, Kragujevac, Serbia. Electronic address: c_danijela@yahoo.com. 2. University of Kragujevac, Faculty of Science, Institute of Biology and Ecology, Kragujevac, Serbia. Electronic address: zivanovicmkg@gmail.com. 3. University of Kragujevac, Faculty of Science, Institute of Biology and Ecology, Kragujevac, Serbia. Electronic address: milenagen@gmail.com. 4. University of Kragujevac, Faculty of Engineering, Kragujevac, Serbia; BioIRC Bioengineering R&D Center, Kragujevac, Serbia. Electronic address: tijana@kg.ac.rs. 5. University of Kragujevac, Faculty of Engineering, Kragujevac, Serbia; BioIRC Bioengineering R&D Center, Kragujevac, Serbia. Electronic address: mradovic@kg.ac.rs. 6. University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia. Electronic address: draleksandarcvetkovic@gmail.com. 7. University of Kragujevac, Faculty of Engineering, Kragujevac, Serbia; University of Harvard, Boston, USA; BioIRC Bioengineering R&D Center, Kragujevac, Serbia. Electronic address: fica@kg.ac.rs. 8. University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia. Electronic address: nzdravkovic@medf.kg.ac.rs.
Abstract
PURPOSE: To study the effects of electroporation on different cell lines. MATERIAL: The effects of electroporation on human breast cancer (MDA-MB-231), human colon cancer (SW-480 and HCT-116), human fibroblast cell line (MRC-5), primary human aortic smooth muscle cells (hAoSMC) and human umbilical vein endothelial cells (HUVEC) were studied. Real-time technology was used for cell viability monitoring. Acridine orange/ethidium bromide assay was applied for cell death type determination. A numerical model of electroporation has been proposed. RESULTS: Electroporation induced inhibition of cell viability on dose (voltage) dependent way. The electroporation treatment 375-437.5Vcm-1 caused irreversible electroporation of cancer cells and reversible electroporation of healthy cells. The application of lower voltage rating (250Vcm-1) led to apoptosis as the predominant type of cell death, whereas the use of higher voltage (500Vcm-1) mainly caused necrosis. CONCLUSION: Electroporation represents a promising method in cancer treatment. Different cancer cell lines had different response to the identical electroporation treatment. Electroporation 375-437.5Vcm-1 selectively caused permanent damage of cancer cells (SW-480), while healthy cells (MRC-5, hAoSM and HUVEC) recovered after 72h. The type of cell death is dependent of electroporation conditions. The proposed numerical model is useful for the analysis of phenomena related to electroporation treatment.
PURPOSE: To study the effects of electroporation on different cell lines. MATERIAL: The effects of electroporation on humanbreast cancer (MDA-MB-231), humancolon cancer (SW-480 and HCT-116), human fibroblast cell line (MRC-5), primary human aortic smooth muscle cells (hAoSMC) and human umbilical vein endothelial cells (HUVEC) were studied. Real-time technology was used for cell viability monitoring. Acridine orange/ethidium bromide assay was applied for cell death type determination. A numerical model of electroporation has been proposed. RESULTS: Electroporation induced inhibition of cell viability on dose (voltage) dependent way. The electroporation treatment 375-437.5Vcm-1 caused irreversible electroporation of cancer cells and reversible electroporation of healthy cells. The application of lower voltage rating (250Vcm-1) led to apoptosis as the predominant type of cell death, whereas the use of higher voltage (500Vcm-1) mainly caused necrosis. CONCLUSION: Electroporation represents a promising method in cancer treatment. Different cancer cell lines had different response to the identical electroporation treatment. Electroporation 375-437.5Vcm-1 selectively caused permanent damage of cancer cells (SW-480), while healthy cells (MRC-5, hAoSM and HUVEC) recovered after 72h. The type of cell death is dependent of electroporation conditions. The proposed numerical model is useful for the analysis of phenomena related to electroporation treatment.
Authors: M Kyle Cromer; Sriram Vaidyanathan; Daniel E Ryan; Bo Curry; Anne Bergstrom Lucas; Joab Camarena; Milan Kaushik; Sarah R Hay; Renata M Martin; Israel Steinfeld; Rasmus O Bak; Daniel P Dever; Ayal Hendel; Laurakay Bruhn; Matthew H Porteus Journal: Mol Ther Date: 2018-07-11 Impact factor: 11.454
Authors: Kang Won Lee; Jae Min Lee; Hyuk Soon Choi; Eun Sun Kim; Bora Keum; Yeon Seok Seo; Yoon Tae Jeen; Soon Ho Um; Hong Sik Lee; Hoon Jai Chun; Chang Duck Kim; Chi Hyuk Oh; Hong Bae Kim Journal: Clin Endosc Date: 2020-10-20