Xianjuan Shen1, Mei Wang2, Yuehua Guo2, Shaoqing Ju1. 1. Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, China. 2. Medical Office, Nantong University, China.
Abstract
BACKGROUND: Lymphoma is a malignant tumor of the immune system originating from lymph nodes and extralymphatic tissues. Its occurrence is believed to be associated with various immune cells due to the proliferation and differentiation of lymphocytes during the immune response. It has been found in many studies that B-cell activating factor (BAFF), as a member of the tumor necrosis factor (TNF) superfamily, could specifically activate B lymphocytes and promote their proliferation. OBJECTIVES: To explore correlations between non-Hodgkin lymphoma (NHL) and the expression of BAFF and its receptors in NHL patients. MATERIAL AND METHODS: The protein expression of BAFF and its receptors in serum and BAFF mRNA expression in peripheral blood mononuclear cells (PBMCs) of 47 NHL patients and 20 healthy subjects were detected by ELISA and RFQ-PCR and compared with LDH and β2M levels. RESULTS: BAFF mRNA expression in the PBMCs of NHL patients was significantly higher than in healthy controls. The expression levels of serum BAFF and the three receptors (TACI, BCMA and BAFF-R) in NHL patients were significantly higher than in healthy controls, and were not significantly correlated with β2M and LDH levels. CONCLUSIONS: The serum protein concentration of BAFF and the expression level of BAFF mRNA in PBMCs of NHL patients underwent abnormal changes, indicating that BAFF and its receptors may play some role in the pathogenesis of NHL.
BACKGROUND:Lymphoma is a malignant tumor of the immune system originating from lymph nodes and extralymphatic tissues. Its occurrence is believed to be associated with various immune cells due to the proliferation and differentiation of lymphocytes during the immune response. It has been found in many studies that B-cell activating factor (BAFF), as a member of the tumor necrosis factor (TNF) superfamily, could specifically activate B lymphocytes and promote their proliferation. OBJECTIVES: To explore correlations between non-Hodgkin lymphoma (NHL) and the expression of BAFF and its receptors in NHLpatients. MATERIAL AND METHODS: The protein expression of BAFF and its receptors in serum and BAFF mRNA expression in peripheral blood mononuclear cells (PBMCs) of 47 NHLpatients and 20 healthy subjects were detected by ELISA and RFQ-PCR and compared with LDH and β2M levels. RESULTS:BAFF mRNA expression in the PBMCs of NHLpatients was significantly higher than in healthy controls. The expression levels of serum BAFF and the three receptors (TACI, BCMA and BAFF-R) in NHLpatients were significantly higher than in healthy controls, and were not significantly correlated with β2M and LDH levels. CONCLUSIONS: The serum protein concentration of BAFF and the expression level of BAFF mRNA in PBMCs of NHLpatients underwent abnormal changes, indicating that BAFF and its receptors may play some role in the pathogenesis of NHL.
Authors: Hong Qin; Zhenyuan Dong; Xiuli Wang; Wesley A Cheng; Feng Wen; Weili Xue; Han Sun; Miriam Walter; Guowei Wei; D Lynne Smith; Xiuhua Sun; Fan Fei; Jianming Xie; Theano I Panagopoulou; Chun-Wei Chen; Joo Y Song; Ibrahim Aldoss; Clarisse Kayembe; Luisa Sarno; Markus Müschen; Giorgio G Inghirami; Stephen J Forman; Larry W Kwak Journal: Sci Transl Med Date: 2019-09-25 Impact factor: 17.956