Literature DB >> 2802623

Purification and characterization of an alpha-D-glucuronidase from a thermophilic fungus, Thermoascus aurantiacus.

K M Khandke1, P J Vithayathil, S K Murthy.   

Abstract

An alpha-D-glucuronidase was purified from the culture filtrates of Thermoascus aurantiacus. A simple colorimetric method for its assay is reported. The enzyme is a single polypeptide chain with a molecular weight of 118,000. It acts optimally at pH 4.5. It shows maximum activity at 65 degrees C. The t 1/2 at 70 degrees C was 40 min. It specifically cleaved the alpha-(1----2) linkage between 4-O-methyl-alpha-D-glucuronic acid and the xylose residue in xylan and several glucurono-xylooligosaccharides.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2802623     DOI： 10.1016/0003-9861(89)90464-5

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  12 in total

Review 1.  Thermophilic fungi: their physiology and enzymes.

Authors:  R Maheshwari; G Bharadwaj; M K Bhat
Journal:  Microbiol Mol Biol Rev       Date:  2000-09       Impact factor: 11.056

2.  Isolation of α-glucuronidase enzyme from a rumen metagenomic library.

Authors:  Charles C Lee; Rena E Kibblewhite; Kurt Wagschal; Ruiping Li; William J Orts
Journal:  Protein J       Date:  2012-03       Impact factor: 2.371

Review 3.  Thermophilic Degradation of Hemicellulose, a Critical Feedstock in the Production of Bioenergy and Other Value-Added Products.

Authors:  Isaac Cann; Gabriel V Pereira; Ahmed M Abdel-Hamid; Heejin Kim; Daniel Wefers; Boniface B Kayang; Tamotsu Kanai; Takaaki Sato; Rafael C Bernardi; Haruyuki Atomi; Roderick I Mackie
Journal:  Appl Environ Microbiol       Date:  2020-03-18       Impact factor: 4.792

Review 4.  Thermostable enzymes as biocatalysts in the biofuel industry.

Authors:  Carl J Yeoman; Yejun Han; Dylan Dodd; Charles M Schroeder; Roderick I Mackie; Isaac K O Cann
Journal:  Adv Appl Microbiol       Date:  2010-03-06       Impact factor: 5.086

5.  alpha-Glucuronidase and Other Hemicellulase Activities of Fibrobacter succinogenes S85 Grown on Crystalline Cellulose or Ball-Milled Barley Straw.

Authors:  D C Smith; C W Forsberg
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

6.  The membrane-bound alpha-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan.

Authors:  Tibor Nagy; Kaveh Emami; Carlos M G A Fontes; Luis M A Ferreira; David R Humphry; Harry J Gilbert
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

7.  Purification and Characterization of the (alpha)-Glucuronidase from Thermoanaerobacterium sp. Strain JW/SL-YS485, an Important Enzyme for the Utilization of Substituted Xylans.

Authors:  W Shao; S Obi; J Puls; J Wiegel
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

8.  Aldouronate utilization in Paenibacillus sp. strain JDR-2: Physiological and enzymatic evidence for coupling of extracellular depolymerization and intracellular metabolism.

Authors:  Guang Nong; John D Rice; Virginia Chow; James F Preston
Journal:  Appl Environ Microbiol       Date:  2009-04-24       Impact factor: 4.792

9.  Alpha-(4-O-methyl)-D-glucuronidase activity produced by the rumen anaerobic fungus Piromonas communis: a study of selected properties.

Authors:  T M Wood; C A Wilson
Journal:  Appl Microbiol Biotechnol       Date:  1995-10       Impact factor: 4.813

10.  Effect of dimer dissociation on activity and thermostability of the alpha-glucuronidase from Geobacillus stearothermophilus: dissecting the different oligomeric forms of family 67 glycoside hydrolases.

Authors:  Dalia Shallom; Gali Golan; Gil Shoham; Yuval Shoham
Journal:  J Bacteriol       Date:  2004-10       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.