X Argemi1, G Prévost2, P Riegel2, D Keller3, N Meyer4, M Baldeyrou5, N Douiri5, N Lefebvre5, K Meghit6, C Ronde Oustau7, D Christmann8, S Cianférani9, J M Strub9, Y Hansmann8. 1. Hôpitaux Universitaires, Maladies Infectieuses et Tropicales, Strasbourg, France; Université de Strasbourg, CHRU de Strasbourg, VBP EA7290, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Institut de Bactériologie, 3 Rue Koeberlé, Strasbourg, France. Electronic address: xavier.argemi@chru-strasbourg.fr. 2. Université de Strasbourg, CHRU de Strasbourg, VBP EA7290, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Institut de Bactériologie, 3 Rue Koeberlé, Strasbourg, France; Hôpitaux Universitaires, Laboratoire de Microbiologie, Strasbourg, France. 3. Hôpitaux Universitaires, Laboratoire de Microbiologie, Strasbourg, France. 4. Hôpitaux Universitaires, Service de Santé Publique, Strasbourg, France. 5. Hôpitaux Universitaires, Maladies Infectieuses et Tropicales, Strasbourg, France. 6. Hôpitaux Universitaires, Immunologie Clinique, Strasbourg, France. 7. Hôpitaux Universitaires, Service de Chirurgie Orthopédique, CCOM, Strasbourg, France. 8. Hôpitaux Universitaires, Maladies Infectieuses et Tropicales, Strasbourg, France; Université de Strasbourg, CHRU de Strasbourg, VBP EA7290, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Institut de Bactériologie, 3 Rue Koeberlé, Strasbourg, France. 9. Laboratoire de Spectrométrie de Masse Bio-Organique, Département des Sciences Analytiques, Institut Pluridisciplinaire Hubert Curien, UMR 7178 (CNRS-UdS), Strasbourg, France.
Abstract
OBJECTIVE: Staphylococcus lugdunensis is a coagulase-negative staphylococcus that displays an unusually high virulence rate close to that of Staphylococcus aureus. It also shares phenotypic properties with S. aureus and several studies found putative virulence factors. The objective of the study was to describe the clinical manifestations of S. lugdunensis infections and investigate putative virulence factors. METHOD: We conducted a prospective study from November 2013 to March 2016 at the University Hospital of Strasbourg. Putative virulence factors were investigated by clumping factor detection, screening for proteolytic activity, and sequence analysis using tandem nano-liquid chromatography-mass spectrometry. RESULTS: In total, 347 positive samples for S. lugdunensis were collected, of which 129 (37.2%) were from confirmed cases of S. lugdunensis infection. Eighty-one of these 129 patients were included in the study. Bone and prosthetic joints (PJI) were the most frequent sites of infection (n=28; 34.6%) followed by skin and soft tissues (n=23; 28.4%). We identified and purified a novel protease secreted by 50 samples (61.7%), most frequently associated with samples from deep infections and PJI (pr 0.97 and pr 0.91, respectively). Protease peptide sequencing by nano-liquid chromatography-mass spectrometry revealed a novel protease bearing 62.42% identity with ShpI, a metalloprotease secreted by Staphylococcus hyicus. CONCLUSION: This study confirms the pathogenicity of S. lugdunensis, particularly in bone and PJI. We also identified a novel metalloprotease called lugdulysin that may contribute to virulence.
OBJECTIVE:Staphylococcus lugdunensis is a coagulase-negative staphylococcus that displays an unusually high virulence rate close to that of Staphylococcus aureus. It also shares phenotypic properties with S. aureus and several studies found putative virulence factors. The objective of the study was to describe the clinical manifestations of S. lugdunensis infections and investigate putative virulence factors. METHOD: We conducted a prospective study from November 2013 to March 2016 at the University Hospital of Strasbourg. Putative virulence factors were investigated by clumping factor detection, screening for proteolytic activity, and sequence analysis using tandem nano-liquid chromatography-mass spectrometry. RESULTS: In total, 347 positive samples for S. lugdunensis were collected, of which 129 (37.2%) were from confirmed cases of S. lugdunensis infection. Eighty-one of these 129 patients were included in the study. Bone and prosthetic joints (PJI) were the most frequent sites of infection (n=28; 34.6%) followed by skin and soft tissues (n=23; 28.4%). We identified and purified a novel protease secreted by 50 samples (61.7%), most frequently associated with samples from deep infections and PJI (pr 0.97 and pr 0.91, respectively). Protease peptide sequencing by nano-liquid chromatography-mass spectrometry revealed a novel protease bearing 62.42% identity with ShpI, a metalloprotease secreted by Staphylococcus hyicus. CONCLUSION: This study confirms the pathogenicity of S. lugdunensis, particularly in bone and PJI. We also identified a novel metalloprotease called lugdulysin that may contribute to virulence.
Authors: Denny Chin; Ronald S Flannagan; Stephen W Tuffs; Jeremy K Chan; John K McCormick; David E Heinrichs Journal: Infect Immun Date: 2022-09-07 Impact factor: 3.609
Authors: Joanne S K Teh; Ioanna Pantelis; Xiao Chen; Tania Sadlon; Kelly Papanaoum; David L Gordon Journal: J Clin Microbiol Date: 2021-10-27 Impact factor: 11.677