| Literature DB >> 28017692 |
M Rahnama1, N Forester2, K G S U Ariyawansa2, C R Voisey2, L J Johnson2, R D Johnson2, D J Fleetwood3.
Abstract
A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the method resulted in 33-74% targeted deletions in E. festucae using as little as 1.5kb of targeting sequence.Entities:
Keywords: Endophyte; Epichloë; Functional analysis; Gene deletion; Split-marker
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Year: 2016 PMID： 28017692 DOI： 10.1016/j.mimet.2016.12.017
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363