Literature DB >> 2801329

Phospholipase A2 (PLA2) activity in rabbit chondrocytes.

T M Stevens1, J E Chin, M McGowan, J Giannaras, J S Kerr.   

Abstract

The effects of recombinant interleukin-1 beta (rIL-1 beta), recombinant tumor necrosis factor (rTNF alpha) and two growth factors, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on PLA2 activity and prostaglandin E2 (PGE2) release were investigated using rabbit chondrocytes. Cellular PLA2 activity increased 2-10 x above controls in the presence of 8 x 10(-12) M (5 U) rIL-1 beta or 5 x 10(-9) M rTNF alpha after 20 hr incubation. PLA2 activity remained constant with 1-50 ng/ml of either growth factor. PGE2 release significantly increased (p less than 0.05) when the chondrocytes were incubated with rIL-1 beta, bFGF and EGF alone, but not with rTNF alpha above. These data suggest PLA2 activity and PGE2 release are not coordinately regulated in rabbit chondrocytes.

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Year:  1989        PMID： 2801329     DOI： 10.1007/bf01972829

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  8 in total

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Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Interleukin-1 and tumor necrosis factor are not synergistic for human synovial fibroblast PLA2 activation and PGE2 production.

Authors:  R W Godfrey; W J Johnson; T Newman; S T Hoffstein
Journal:  Prostaglandins       Date:  1988-01

3.  Phospholipases and phospholipid turnover in Escherichia coli spheroplasts.

Authors:  P Patriarca; S Beckerdite; P Elsbach
Journal:  Biochim Biophys Acta       Date:  1972-04-18

4.  Activation of rabbit articular chondrocytes by recombinant human cytokines.

Authors:  S C Gilman
Journal:  J Rheumatol       Date:  1987-10       Impact factor: 4.666

5.  Interleukin 2-independent stimulation of rabbit chondrocyte collagenase and prostaglandin E2 production by an interleukin 1-like factor.

Authors:  V Evêquoz; F Bettens; F Kristensen; U Trechsel; B M Stadler; J M Dayer; A L De Weck; H Fleisch
Journal:  Eur J Immunol       Date:  1984-06       Impact factor: 5.532

6.  Interleukin 1 activates phospholipase A2 in rabbit chondrocytes: a possible signal for IL 1 action.

Authors:  J Chang; S C Gilman; A J Lewis
Journal:  J Immunol       Date:  1986-02-15       Impact factor: 5.422

7.  Phospholipase A2 activation by interleukin 1: release and metabolism of arachidonic acid by IL 1-stimulated rabbit chondrocytes.

Authors:  S C Gilman; P R Berner; J Chang
Journal:  Agents Actions       Date:  1987-08

8.  Inhibition of phospholipase A2 by "lipocortins" and calpactins. An effect of binding to substrate phospholipids.

Authors:  F F Davidson; E A Dennis; M Powell; J R Glenney
Journal:  J Biol Chem       Date:  1987-02-05       Impact factor: 5.157
  8 in total
  2 in total

1.  Phospholipases of mineralization competent cells and matrix vesicles: roles in physiological and pathological mineralizations.

Authors:  Saida Mebarek; Abdelkarim Abousalham; David Magne; Le Duy Do; Joanna Bandorowicz-Pikula; Slawomir Pikula; René Buchet
Journal:  Int J Mol Sci       Date:  2013-03-01       Impact factor: 5.923

2.  Immunolocalization of basic fibroblast growth factor and platelet-derived growth factor-A during adjuvant arthritis in the Lewis rat.

Authors:  Z Qu; M Picou; T T Dang; E Angell; S R Planck; C E Hart; J T Rosenbaum
Journal:  Am J Pathol       Date:  1994-11       Impact factor: 4.307
  2 in total

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