| Literature DB >> 28013043 |
Jacinto Gomes1, Marcos Santos2, Ana Amaro3, Isabel Pereira da Fonseca2, Gabriela Santos-Gomes4, João Inácio5.
Abstract
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.Entities:
Keywords: Bovine theileriosis; LAMP; Molecular diagnostics; Real-time PCR; Tams1; Theileria annulata
Mesh：
Year: 2016 PMID： 28013043 DOI： 10.1016/j.mcp.2016.12.006
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365