| Literature DB >> 28012155 |
Victoria V Kiroshka1, Valentina A Petrova2, Daniil D Chernyakov2, Yulia O Bozhkova1, Katerina V Kiroshka1, Yulia G Baklagina2, Dmitry P Romanov3, Roman V Kremnev4, Yury A Skorik5.
Abstract
Chitosan scaffolds have gained much attention in various tissue engineering applications, but the effect of their microstructure on cell-material spatial interactions remains unclear. Our objective was to evaluate the effect of chitosan-based matrices doping with chitin nano-whiskers (CNW) on adhesion, spreading, cytoskeleton structure, and proliferation of rat bone marrow stromal cells (BMSCs). The behavior of BMSCs during culture on chitosan-CNW films was determined by the molecular mass, hydrophobicity, porosity, crosslinking degree, protonation degree and molecular structure of the composite chitosan-CNW films. The shape, spreading area, cytoskeleton structure, and proliferation of BMSCs on chitosan matrices with a crystalline structure and high porosity were similar to that observed for BMSCs cultured on polystyrene tissue culture plates. The amorphous polymer structure and high swelling led to a decrease in the spreading area and cell proliferation. Thus, we can control the behavior of cells in culture (adhesion, spreading, and proliferation) by changing the physico-chemical properties of the chitosan-CNW films.Entities:
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Year: 2016 PMID： 28012155 DOI： 10.1007/s10856-016-5822-2
Source DB: PubMed Journal: J Mater Sci Mater Med ISSN： 0957-4530 Impact factor: 3.896