Takeharu Imai1,2, Naohide Oue3, Kazuhiro Sentani1, Naoya Sakamoto1, Naohiro Uraoka1, Hiroyuki Egi4, Takao Hinoi4,5, Hideki Ohdan4, Kazuhiro Yoshida2, Wataru Yasui1. 1. Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan. 2. Department of Surgical Oncology, Graduate School of Medicine, Gifu University, Gifu, Japan. 3. Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan naoue@hiroshima-u.ac.jp. 4. Department of Gastroenterological and Transplant Surgery, Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. 5. Department of Surgery, Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chu-goku Cancer Center, Kure, Japan.
Abstract
BACKGROUND: Oesophageal squamous cell carcinoma (ESCC) and colorectal cancer (CRC) are common types of human cancer. Spheroid colony formation is used to characterize cancer stem cell (CSCs). In the present study, we analyzed the significance of kinesin family 11 (KIF11 in human ESCC and CRC. MATERIALS AND METHODS: Expression of KIF11 in 105 ESCC and 100 CRC cases was determined using immunohistochemistry. RNA interference was used to inhibit KIF11 expression in ESCC and CRC cell lines. RESULTS: In total, 61 out of 105 (58%) ESCC and 62 out of 100 (62%) CRC cases were positive for KIF11. Expression of KIF11 was not associated with any clinicopathological characteristics. Both the number and size of spheres produced by from TE-5 ESCC cells and DLD-1 CRC cells were significantly reduced upon KIF11 siRNA transfection compared to negative control siRNA transfection. CONCLUSION: These results indicate that KIF11 plays an important role in CSCs of ESCC and CRC. Copyright
BACKGROUND:Oesophageal squamous cell carcinoma (ESCC) and colorectal cancer (CRC) are common types of humancancer. Spheroid colony formation is used to characterize cancer stem cell (CSCs). In the present study, we analyzed the significance of kinesin family 11 (KIF11 in human ESCC and CRC. MATERIALS AND METHODS: Expression of KIF11 in 105 ESCC and 100 CRC cases was determined using immunohistochemistry. RNA interference was used to inhibit KIF11 expression in ESCC and CRC cell lines. RESULTS: In total, 61 out of 105 (58%) ESCC and 62 out of 100 (62%) CRC cases were positive for KIF11. Expression of KIF11 was not associated with any clinicopathological characteristics. Both the number and size of spheres produced by from TE-5 ESCC cells and DLD-1 CRC cells were significantly reduced upon KIF11 siRNA transfection compared to negative control siRNA transfection. CONCLUSION: These results indicate that KIF11 plays an important role in CSCs of ESCC and CRC. Copyright
Authors: Bruna Cândido Guido; Douglas Cardoso Brandão; Ana Luisa Augusto Barbosa; Monique Jacob Xavier Vianna; Lucas Faro; Luciana Machado Ramos; Fabíola Nihi; Márcio Botelho de Castro; Brenno A D Neto; José Raimundo Corrêa; Sônia Nair Báo Journal: Oncotarget Date: 2021-09-14