| Literature DB >> 28010109 |
Akira Muramatsu1, Yuta Shimizu1, Yuko Yoshikawa1, Wakao Fukuda2, Naoki Umezawa3, Yuhei Horai3, Tsunehiko Higuchi3, Shinsuke Fujiwara4, Tadayuki Imanaka2, Kenichi Yoshikawa1.
Abstract
We studied the effect of branched-chain polyamines on the folding transition of genome-sized DNA molecules in aqueous solution by the use of single-molecule observation with fluorescence microcopy. Detailed morphological features of polyamine/DNA complexes were characterized by atomic force microscopy (AFM). The AFM observations indicated that branched-chain polyamines tend to induce a characteristic change in the higher-order structure of DNA by forming bridges or crosslinks between the segments of a DNA molecule. In contrast, natural linear-chain polyamines cause a parallel alignment between DNA segments. Circular dichroism measurements revealed that branched-chain polyamines induce the A-form in the secondary structure of DNA, while linear-chain polyamines have only a minimum effect. This large difference in the effects of branched- and linear-chain polyamines is discussed in relation to the difference in the manner of binding of these polyamines to negatively charged double-stranded DNA.Entities:
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Year: 2016 PMID： 28010109 DOI： 10.1063/1.4972066
Source DB: PubMed Journal: J Chem Phys ISSN： 0021-9606 Impact factor: 3.488