Nucleotide sequence of E1 region of canine adenovirus type 2.

The nucleotide sequence of the leftmost EcoRI-C fragment (0 to 11.3%) of canine adenovirus type 2 (CAd2) which could transform rodent cells morphologically but required additional sequences from 10 to 32 map units (m.u.) for full expression of its oncogenic potential was determined. The EcoRI-C fragment contains 3609 nucleotide base pairs (bp) encoding E1A, E1B, and pIX genes. Although the nucleotide sequence of CAd2 E1 shows little homology to those of human Ads, the amino acid sequences of the E1 proteins predicted from nucleotide sequence of CAd2 E1 and those for human and simian Ads are partially conserved.