Eric A Legenzov1, Sukumaran Muralidharan1, Lukas B Woodcock2, Gareth R Eaton2, Sandra S Eaton2, Gerald M Rosen3, Joseph P Y Kao1. 1. Center for Biomedical Engineering & Technology, and Department of Physiology, University of Maryland School of Medicine , Baltimore, Maryland 21201, United States. 2. Department of Chemistry and Biochemistry, University of Denver , Denver, Colorado 80208, United States. 3. Department of Pharmaceutical Sciences, School of Pharmacy, and Center for Biomedical Engineering & Technology, School of Medicine, University of Maryland , Baltimore, Maryland 21201, United States.
Abstract
Targeted delivery of molecular probes into cells enables cellular imaging through optical and magnetic modalities. Probe molecules that are well retained by cells can accumulate to higher intracellular concentrations, and thus increase the signal-to-noise ratio of, and widen the temporal window for, imaging. Here we synthesize a paramagnetic spin probe bearing six ionic functional groups and show that it has long intracellular half-life (>12 h) and exceptional biostability in living cells. We demonstrate that judicious incorporation of ionic substituents on probe molecules systematically increases intracellular retention time, and should therefore be beneficial to imaging experiments.
Targeted delivery of molecular probes into cells enables cellular imaging through optical and magnetic modalities. Probe molecules that are well retained by cells can accumulate to higher intracellular concentrations, and thus increase the signal-to-noise n class="Species">ratio of, and widen the temporal window for, imaging. Here we synthesize a paramagnetic spin probe bearing six ionic functional groups and show that it has long intracellular half-life (>12 h) and exceptional biostability in living cells. We demonstrate that judicious incorporation of ionic substituents on probe molecules systematically increases intracellular retention time, and should therefore be beneficial to imaging experiments.
Authors: Scott R Burks; Luciana F Macedo; Eugene D Barth; Katherine H Tkaczuk; Stuart S Martin; Gerald M Rosen; Howard J Halpern; Angela M Brodie; Joseph P Y Kao Journal: Breast Cancer Res Treat Date: 2010-01-12 Impact factor: 4.872