Sinem Sarı Gökay1, Rıza Dinçer Yıldızdaş2, Mustafa Yılmaz1, Kıymet Aksoy3, Ali Erdinç Yalın3, Yaşar Sertdemir4, Gülsüm Uçar5, Özden Özgür Horoz2, Fatma Derya Özduran1, Hayri Levent Yılmaz1. 1. Deparment of Pediatrics, Çukurova University School of Medicine, Adana, Turkey. 2. Deparment of Pediatric Intensive Care, Çukurova University School of Medicine, Adana, Turkey. 3. Department of Biochemistry, Çukurova University School of Medicine, Adana, Turkey. 4. Department of Biostatistics, Çukurova University School of Medicine, Adana, Turkey. 5. Department of Pediatric Immunology Laboratory, Çukurova University School of Medicine, Adana, Turkey.
Abstract
BACKGROUND: Myeloid differentiation primary response gene 88 (MyD 88) is an intracellular adapter protein that mediates the early immune response to pathogens. Toll-like receptors (except TLR-3) induce the immune response through a MyD 88-dependent signal pathway. AIMS: We aimed to investigate the MyD 88 polymorphisms that play important roles in the immune response in septic children and to evaluate whether or not they were risk factors in the development of sepsis. STUDY DESIGN: Case-control study. METHODS: Sixty-five patients diagnosed with sepsis in the Pediatric Intensive Care Unit during the period from April 2010 to January 2012 were included as the study group. Sixty-five children without sepsis were included as controls. After DNA was obtained from blood samples in the study and control groups, MyD 88 polymorphisms were analyzed. According to the genotype and allele frequencies, the distributions of MyD 88 polymorphisms [Single nucleotide polymorphism (SNP) - 938 C/A (rs4988453), MyD 88 SNP 1944 C/G (rs4988457)] were analyzed in both the study and control groups. RESULTS: The C/C genotype of MyD 88 SNP -938 was significantly more common than the C/A genotype in the patient group (p=0.002). No statistically significant difference in the frequency of the MyD 88 SNP 1944 genotype was found between the study and control groups (p=0.272). CONCLUSION: Gene polymorphism studies could elucidate our understanding of sepsis in terms of prevalence and the management of treatment. It was shown in this study that children with the MyD 88 SNP -938 C/C genotype had a greater tendency toward sepsis. However, additional studies should be performed.
BACKGROUND: Myeloid differentiation primary response gene 88 (MyD 88) is an intracellular adapter protein that mediates the early immune response to pathogens. Toll-like receptors (except TLR-3) induce the immune response through a MyD 88-dependent signal pathway. AIMS: We aimed to investigate the MyD 88 polymorphisms that play important roles in the immune response in septic children and to evaluate whether or not they were risk factors in the development of sepsis. STUDY DESIGN: Case-control study. METHODS: Sixty-five patients diagnosed with sepsis in the Pediatric Intensive Care Unit during the period from April 2010 to January 2012 were included as the study group. Sixty-five children without sepsis were included as controls. After DNA was obtained from blood samples in the study and control groups, MyD 88 polymorphisms were analyzed. According to the genotype and allele frequencies, the distributions of MyD 88 polymorphisms [Single nucleotide polymorphism (SNP) - 938 C/A (rs4988453), MyD 88 SNP 1944 C/G (rs4988457)] were analyzed in both the study and control groups. RESULTS: The C/C genotype of MyD 88 SNP -938 was significantly more common than the C/A genotype in the patient group (p=0.002). No statistically significant difference in the frequency of the MyD 88 SNP 1944 genotype was found between the study and control groups (p=0.272). CONCLUSION: Gene polymorphism studies could elucidate our understanding of sepsis in terms of prevalence and the management of treatment. It was shown in this study that children with the MyD 88 SNP -938 C/C genotype had a greater tendency toward sepsis. However, additional studies should be performed.
Authors: Theo S Plantinga; Melissa D Johnson; William K Scott; Esther van de Vosse; Digna R Velez Edwards; P Brian Smith; Barbara D Alexander; John C Yang; Dennis Kremer; Gregory M Laird; Marije Oosting; Leo A B Joosten; Jos W M van der Meer; Jaap T van Dissel; Thomas J Walsh; John R Perfect; Bart Jan Kullberg; Mihai G Netea Journal: J Infect Dis Date: 2012-02-01 Impact factor: 5.226
Authors: Capucine Picard; Horst von Bernuth; Pegah Ghandil; Maya Chrabieh; Ofer Levy; Peter D Arkwright; Douglas McDonald; Raif S Geha; Hidetoshi Takada; Jens C Krause; C Buddy Creech; Cheng-Lung Ku; Stephan Ehl; László Maródi; Saleh Al-Muhsen; Sami Al-Hajjar; Abdulaziz Al-Ghonaium; Noorbibi K Day-Good; Steven M Holland; John I Gallin; Helen Chapel; David P Speert; Carlos Rodriguez-Gallego; Elena Colino; Ben-Zion Garty; Chaim Roifman; Toshiro Hara; Hideto Yoshikawa; Shigeaki Nonoyama; Joseph Domachowske; Andrew C Issekutz; Mimi Tang; Joanne Smart; Simona Eva Zitnik; Cyrille Hoarau; Dinakantha S Kumararatne; Adrian J Thrasher; E Graham Davies; Claire Bethune; Nicolas Sirvent; Dominique de Ricaud; Yildiz Camcioglu; Júlia Vasconcelos; Margarida Guedes; Artur Bonito Vitor; Carlos Rodrigo; Francisco Almazán; Maria Méndez; Juan Ignacio Aróstegui; Laia Alsina; Claudia Fortuny; Janine Reichenbach; James W Verbsky; Xavier Bossuyt; Rainer Doffinger; Laurent Abel; Anne Puel; Jean-Laurent Casanova Journal: Medicine (Baltimore) Date: 2010-11 Impact factor: 1.889