Literature DB >> 27992182

Heparin and Methionine Oxidation Promote the Formation of Apolipoprotein A-I Amyloid Comprising α-Helical and β-Sheet Structures.

David Townsend1, Eleri Hughes1, Rohanah Hussain2, Giuliano Siligardi2, Sarah Baldock1, Jillian Madine3, David A Middleton1.   

Abstract

Peptides derived from apolipoprotein A-I (apoA-I), the main component of high-density lipoprotein (HDL), constitute the main component of amyloid deposits that colocalize with atherosclerotic plaques. Here we investigate the molecular details of full-length, lipid-deprived apoA-I after assembly into insoluble aggregates under physiologically relevant conditions known to induce aggregation in vitro. Unmodified apoA-I is shown to remain soluble at pH 7 for at least 3 days, retaining its native α-helical-rich structure. Upon acidification to pH 4, apoA-I rapidly assembles into insoluble nonfibrillar aggregates lacking the characteristic cross-β features of amyloid. In the presence of heparin, the rate and thioflavin T responsiveness of the aggregates formed at pH 4 increase and short amyloid-like fibrils are observed, which give rise to amyloid-characteristic X-ray reflections at 4.7 and 10 Å. Solid-state nuclear magnetic resonance (SSNMR) and synchrotron radiation circular dichroism spectroscopy of fibrils formed in the presence of heparin show they retain some α-helical characteristics together with new β-sheet structures. Interestingly, SSNMR indicates a similar molecular structure of aggregates formed in the absence of heparin at pH 6 after oxidation of the three methionine residues, although their morphology is rather different from that of the heparin-derived fibrils. We propose a model for apoA-I aggregation in which perturbations of a four-helix bundle-like structure, induced by interactions of heparin or methionine oxidation, cause the partially helical N-terminal residues to disengage from the remaining, intact helices, thereby allowing self-assembly via β-strand associations.

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Year:  2017        PMID: 27992182     DOI: 10.1021/acs.biochem.6b01120

Source DB:  PubMed          Journal:  Biochemistry        ISSN: 0006-2960            Impact factor:   3.162


  13 in total

1.  Learning from Synthetic Models of Extracellular Matrix; Differential Binding of Wild Type and Amyloidogenic Human Apolipoprotein A-I to Hydrogels Formed from Molecules Having Charges Similar to Those Found in Natural GAGs.

Authors:  Silvana A Rosú; Leandro Toledo; Bruno F Urbano; Susana A Sanchez; Graciela C Calabrese; M Alejandra Tricerri
Journal:  Protein J       Date:  2017-08       Impact factor: 2.371

2.  Methionine oxidized apolipoprotein A-I at the crossroads of HDL biogenesis and amyloid formation.

Authors:  Andrzej Witkowski; Gary K L Chan; Jennifer C Boatz; Nancy J Li; Ayuka P Inoue; Jaclyn C Wong; Patrick C A van der Wel; Giorgio Cavigiolio
Journal:  FASEB J       Date:  2018-01-17       Impact factor: 5.191

Review 3.  Redox Signaling by Reactive Electrophiles and Oxidants.

Authors:  Saba Parvez; Marcus J C Long; Jesse R Poganik; Yimon Aye
Journal:  Chem Rev       Date:  2018-08-27       Impact factor: 60.622

4.  High-efficient bacterial production of human ApoA-I amyloidogenic variants.

Authors:  Rita Del Giudice; Jens O Lagerstedt
Journal:  Protein Sci       Date:  2018-12       Impact factor: 6.725

5.  Methoxyphenol derivatives as reversible inhibitors of myeloperoxidase as potential antiatherosclerotic agents.

Authors:  Premkumar Jayaraj; Chandrakala A Narasimhulu; Andrei Maiseyeu; Rekha Durairaj; Shashidhar Rao; Sanjay Rajagopalan; Sampath Parthasarathy; Rajagopal Desikan
Journal:  Future Med Chem       Date:  2019-11-26       Impact factor: 4.767

6.  Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin.

Authors:  David Townsend; Eleri Hughes; Geoffrey Akien; Katie L Stewart; Sheena E Radford; David Rochester; David A Middleton
Journal:  J Biol Chem       Date:  2018-05-31       Impact factor: 5.157

Review 7.  High-Density Lipoprotein in Lupus: Disease Biomarkers and Potential Therapeutic Strategy.

Authors:  Sang Yeop Kim; Minzhi Yu; Emily E Morin; Jukyung Kang; Mariana J Kaplan; Anna Schwendeman
Journal:  Arthritis Rheumatol       Date:  2019-11-26       Impact factor: 10.995

8.  Effect of Phosphatidylserine and Cholesterol on Membrane-mediated Fibril Formation by the N-terminal Amyloidogenic Fragment of Apolipoprotein A-I.

Authors:  Chiharu Mizuguchi; Mitsuki Nakamura; Naoko Kurimitsu; Takashi Ohgita; Kazuchika Nishitsuji; Teruhiko Baba; Akira Shigenaga; Toshinori Shimanouchi; Keiichiro Okuhira; Akira Otaka; Hiroyuki Saito
Journal:  Sci Rep       Date:  2018-04-03       Impact factor: 4.379

9.  Synchrotron radiation circular dichroism spectroscopy reveals structural divergences in HDL-bound apoA-I variants.

Authors:  Rita Del Giudice; Oktawia Nilsson; Joan Domingo-Espín; Jens O Lagerstedt
Journal:  Sci Rep       Date:  2017-10-19       Impact factor: 4.379

10.  BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra.

Authors:  András Micsonai; Frank Wien; Éva Bulyáki; Judit Kun; Éva Moussong; Young-Ho Lee; Yuji Goto; Matthieu Réfrégiers; József Kardos
Journal:  Nucleic Acids Res       Date:  2018-07-02       Impact factor: 16.971

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