M García Bernal1, N Trabal Fernández2, P E Saucedo Lastra3, R Medina Marrero1, J M Mazón-Suástegui3. 1. Centro de Bioactivos Químicos, Universidad Central de Las Villas, Villa Clara, Cuba. 2. Centro Universitario Regional del Este (CURE), Universidad de la República, Rocha, Uruguay. 3. Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), La Paz, Baja California Sur, México.
Abstract
AIM: To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8. METHODS AND RESULTS: DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richness was observed in the initial and final control group, whereas Streptomyces-treated oysters exhibited intermediate values. Proteobacteria was the most abundant phylum (81·4-95·1%), followed by Bacteroidetes, Actinobacteria and Firmicutes. The genera Anderseniella, Oceanicola, Roseovarius, Ruegeria, Sulfitobacter, Granulosicoccus and Marinicella encompassed the core microbiota of all experimental groups. The genus Bacteriovorax was detected in all groups except in the final control and the depurated N7, whereas Vibrio remained undetected in all Streptomyces-treated groups. RL8 was the only group that harboured the genus Streptomyces in its microbiota. Principal component analysis showed that Streptomyces strains significantly changed oyster microbiota with respect to the initial and final control. CONCLUSIONS: Crassostrea sikamea treated with Streptomyces showed high species diversity and a microbiota composition shift, characterized by keeping the predator genus Bacteriovorax and decreasing the pathogenic Vibrio. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first culture-independent study showing the effect of Streptomyces over the oyster microbiota. It also sheds light about the potential use of Streptomyces to improve mollusc health and safety for consumers after the depuration process.
AIM: To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8. METHODS AND RESULTS: DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richness was observed in the initial and final control group, whereas Streptomyces-treated oysters exhibited intermediate values. Proteobacteria was the most abundant phylum (81·4-95·1%), followed by Bacteroidetes, Actinobacteria and Firmicutes. The genera Anderseniella, Oceanicola, Roseovarius, Ruegeria, Sulfitobacter, Granulosicoccus and Marinicella encompassed the core microbiota of all experimental groups. The genus Bacteriovorax was detected in all groups except in the final control and the depurated N7, whereas Vibrio remained undetected in all Streptomyces-treated groups. RL8 was the only group that harboured the genus Streptomyces in its microbiota. Principal component analysis showed that Streptomyces strains significantly changed oyster microbiota with respect to the initial and final control. CONCLUSIONS:Crassostrea sikamea treated with Streptomyces showed high species diversity and a microbiota composition shift, characterized by keeping the predator genus Bacteriovorax and decreasing the pathogenic Vibrio. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first culture-independent study showing the effect of Streptomyces over the oyster microbiota. It also sheds light about the potential use of Streptomyces to improve mollusc health and safety for consumers after the depuration process.
Authors: Lauren K Redfern; Nishad Jayasundara; David R Singleton; Richard T Di Giulio; James Carlson; Susan J Sumner; Claudia K Gunsch Journal: Sci Total Environ Date: 2021-02-19 Impact factor: 10.753
Authors: José Manuel Mazón-Suástegui; Joan Sebastian Salas-Leiva; Ricardo Medina-Marrero; Ricardo Medina-García; Milagro García-Bernal Journal: Microbiologyopen Date: 2019-11-18 Impact factor: 3.139
Authors: Marcos Vinícius Reis Conceição; Sávio Souza Costa; Ana Paula Schaan; Ândrea Kely Campos Ribeiro-Dos-Santos; Artur Silva; Diego Assis das Graças; Maria Paula Cruz Schneider; Rafael Azevedo Baraúna Journal: Front Genet Date: 2021-02-12 Impact factor: 4.599