Li-Lun Wang1, Hai-Feng Hu2, Yan-Qin Feng1. 1. Department of Ophthalmology, Affiliated Hospital of Yan'an University, Yan'an 716000, Shaanxi Province, China. 2. Department of Oncology, Affiliated Hospital of Yan'an University, Yan'an 716000, Shaanxi Province, China.
Abstract
AIM: To investigate microRNA-143 expression and effect on suppression of retinoblastoma (RB) cells. METHODS: The expression of microRNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1. The microRNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods. RESULTS: The microRNA-143 expression was significantly suppressed in RB cell lines. Overexpression of microRNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the microRNA-143 mimics transfected group than that in the negative control and the microRNA-143 inhibitor groups. CONCLUSION: MicroRNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.
AIM: To investigate microRNA-143 expression and effect on suppression of retinoblastoma (RB) cells. METHODS: The expression of microRNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1. The microRNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods. RESULTS: The microRNA-143 expression was significantly suppressed in RB cell lines. Overexpression of microRNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the microRNA-143 mimics transfected group than that in the negative control and the microRNA-143 inhibitor groups. CONCLUSION:MicroRNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.
Authors: Femke Simmer; Sabine Venderbosch; Jeroen R Dijkstra; Elisa M Vink-Börger; Claudius Faber; Leonie J Mekenkamp; Miriam Koopman; Anton F De Haan; Cornelis J Punt; Iris D Nagtegaal Journal: Oncotarget Date: 2015-09-08
Authors: Stefano Volinia; George A Calin; Chang-Gong Liu; Stefan Ambs; Amelia Cimmino; Fabio Petrocca; Rosa Visone; Marilena Iorio; Claudia Roldo; Manuela Ferracin; Robyn L Prueitt; Nozumu Yanaihara; Giovanni Lanza; Aldo Scarpa; Andrea Vecchione; Massimo Negrini; Curtis C Harris; Carlo M Croce Journal: Proc Natl Acad Sci U S A Date: 2006-02-03 Impact factor: 11.205