Edison Andrés Cruz Olivo1, Daniel Santos2, Maria Elena de Lima2, Vera Lúcia Dos Santos3, Rubén Dario Sinisterra4, María Esperanza Cortés5. 1. Department of Periodontology, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil. 2. Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais. 3. Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais. 4. Department of Inorganic Chemistry, Exacts Science Institute, Federal University of Minas Gerais. 5. Department of Restorative Dentistry, Faculty of Dentistry, Federal University of Minas Gerais.
Abstract
BACKGROUND: Antimicrobial peptides (AMPs) have shown rapid and potent effect against planktonic bacteria. However, control of periodontopathic biofilms is a challenge even for AMPs. Thus, the present study evaluates in vitro antimicrobial activity of synthetic peptide LyeTxI and association compound LyeTxI/β-cyclodextrin (βCD) against multispecies biofilms. METHODS: Sensibility to LyeTxI and LyeTxI/βCD was determined for planktonic Gram-negative periodontopathogens. Time-kill kinetic assay was performed at minimum inhibitory concentrations (MICs) in all planktonic strains. Multispecies biofilms were grown on pegs using a biofilm device and studied by scanning electron microscopy at 2, 5, and 10 days. Minimal biofilm eradication concentration (MBEC) was determined for 2- and 4-day multispecies biofilms. Metabolic activity of biofilms was determined by fluorometry study. RESULTS: Biofilms showed reproducible cell density on pegs of the biofilm device. LyeTxI and LyeTxI/βCD were active against all strains tested at concentrations ≤62.5 μg/mL. Kinetic assays showed rapid bactericidal effect of LyeTxI against all periodontopathogens. MBECs of LyeTxI and LyeTxI/βCD against multispecies 2-day biofilms were two-fold higher than MICs of cells shed from biofilms. LyeTxI was able to reduce multispecies 2-day metabolic activity by 90%. Multispecies 4-day biofilms were tolerant to all agents tested. CONCLUSIONS: LyeTxI and LyeTxI/βCD are active against periodontopathic bacteria, showing rapid bactericidal effect and may be used to prevent biofilm development. In the future, AMPs could be therapeutic tools for treatment of periodontitis.
BACKGROUND: Antimicrobial peptides (AMPs) have shown rapid and potent effect against planktonic bacteria. However, control of periodontopathic biofilms is a challenge even for AMPs. Thus, the present study evaluates in vitro antimicrobial activity of synthetic peptide LyeTxI and association compound LyeTxI/β-cyclodextrin (βCD) against multispecies biofilms. METHODS: Sensibility to LyeTxI and LyeTxI/βCD was determined for planktonic Gram-negative periodontopathogens. Time-kill kinetic assay was performed at minimum inhibitory concentrations (MICs) in all planktonic strains. Multispecies biofilms were grown on pegs using a biofilm device and studied by scanning electron microscopy at 2, 5, and 10 days. Minimal biofilm eradication concentration (MBEC) was determined for 2- and 4-day multispecies biofilms. Metabolic activity of biofilms was determined by fluorometry study. RESULTS: Biofilms showed reproducible cell density on pegs of the biofilm device. LyeTxI and LyeTxI/βCD were active against all strains tested at concentrations ≤62.5 μg/mL. Kinetic assays showed rapid bactericidal effect of LyeTxI against all periodontopathogens. MBECs of LyeTxI and LyeTxI/βCD against multispecies 2-day biofilms were two-fold higher than MICs of cells shed from biofilms. LyeTxI was able to reduce multispecies 2-day metabolic activity by 90%. Multispecies 4-day biofilms were tolerant to all agents tested. CONCLUSIONS:LyeTxI and LyeTxI/βCD are active against periodontopathic bacteria, showing rapid bactericidal effect and may be used to prevent biofilm development. In the future, AMPs could be therapeutic tools for treatment of periodontitis.
Authors: Pablo V M Reis; Daiane Boff; Rodrigo M Verly; Marcella N Melo-Braga; María E Cortés; Daniel M Santos; Adriano M de C Pimenta; Flávio A Amaral; Jarbas M Resende; Maria E de Lima Journal: Front Microbiol Date: 2018-04-06 Impact factor: 5.640
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Authors: Leonardo Lima Fuscaldi; Joaquim Teixeira de Avelar Júnior; Daniel Moreira Dos Santos; Daiane Boff; Vívian Louise Soares de Oliveira; Karla Aparecida Guimarães Gusmão Gomes; Rosana de Carvalho Cruz; Patrícia Luciana de Oliveira; Paula Prazeres Magalhães; Patricia Silva Cisalpino; Luiz de Macêdo Farias; Elaine Maria de Souza-Fagundes; Johannes Delp; Marcel Leist; Jarbas Magalhães Resende; Flávio Almeida Amaral; Adriano Monteiro de Castro Pimenta; Simone Odília Antunes Fernandes; Valbert Nascimento Cardoso; Maria Elena de Lima Journal: Exp Biol Med (Maywood) Date: 2020-11-11