Literature DB >> 27986353

Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR.

Amelia E Hanron1, Zachary P Billman2, Annette M Seilie1, Ming Chang1, Sean C Murphy3.   

Abstract

Babesia are increasingly appreciated as a cause of transfusion-transmitted infection. Sensitive methods are needed to screen blood products. We report herein that B. microti 18S rRNA is over 1,000-fold more abundant than its coding genes, making reverse transcription PCR (RT-PCR) much more sensitive than PCR. Babesia 18S rRNA may be useful for screening the blood supply.
Copyright © 2016 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  18S rRNA; Babesia; Clinical microbiology; Diagnostics; RT-PCR; Transfusion

Mesh：

Substances：

Year:  2016        PMID： 27986353     DOI： 10.1016/j.diagmicrobio.2016.11.021

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  4 in total

Review 1.  Babesia microti: from Mice to Ticks to an Increasing Number of Highly Susceptible Humans.

Authors:  Lars F Westblade; Matthew S Simon; Blaine A Mathison; Laura A Kirkman
Journal:  J Clin Microbiol       Date:  2017-07-26       Impact factor: 5.948

2.  Untargeted metagenomics shows a reliable performance for synchronous detection of parasites.

Authors:  Claudia Wylezich; Simone M Caccio; Julia Walochnik; Martin Beer; Dirk Höper
Journal:  Parasitol Res       Date:  2020-06-26       Impact factor: 2.289

3.  Beyond Blood Smears: Qualification of Plasmodium 18S rRNA as a Biomarker for Controlled Human Malaria Infections.

Authors:  Annette M Seilie; Ming Chang; Amelia E Hanron; Zachary P Billman; Brad C Stone; Kevin Zhou; Tayla M Olsen; Glenda Daza; Jose Ortega; Kurtis R Cruz; Nahum Smith; Sara A Healy; Jillian Neal; Carolyn K Wallis; Lisa Shelton; Tracie VonGoedert Mankowski; Sharon Wong-Madden; Sebastian A Mikolajczak; Ashley M Vaughan; Stefan H I Kappe; Matt Fishbaugher; Will Betz; Mark Kennedy; Jen C C Hume; Angela K Talley; Stephen L Hoffman; Sumana Chakravarty; B Kim Lee Sim; Thomas L Richie; Anna Wald; Christopher V Plowe; Kirsten E Lyke; Matthew Adams; Gary A Fahle; Elliot P Cowan; Patrick E Duffy; James G Kublin; Sean C Murphy
Journal:  Am J Trop Med Hyg       Date:  2019-06       Impact factor: 2.345

4.  Detection of Babesia RNA and DNA in whole blood samples from US blood donations.

Authors:  Jean Stanley; Susan L Stramer; Yasuko Erickson; Julie Cruz; Jed Gorlin; Mark Janzen; Susan N Rossmann; Todd Straus; Patrick Albrecht; Lisa Lee Pate; Susan A Galel
Journal:  Transfusion       Date:  2021-08-08       Impact factor: 3.337
  4 in total

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