Nilo César do Vale Baracho1, Lucas Miranda Kangussu2, Thiago Ruiz Rodrigues Prestes3, Kátia Daniela da Silveira4, Regina Maria Pereira5, Natália Pessoa Rocha6, Ana Cristina Simões E Silva7. 1. Full Professor, Departament of Biochemistry and Pharmacology, School of Medicine, Itajuba-MG, Brasil. Conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; histopathological examinations; manuscript preparation and writing, final approval. 2. PhD, Interdisciplinary Laboratory of Medical Research, School of Medicine, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brasil. Analysis and interpretation of data, statistical analysis, manuscript writing, final approval. 3. Graduate student, Interdisciplinary Laboratory of Medical Research, School of Medicine, UFMG, Belo Horizonte-MG, Brasil. Technical procedures, manuscript preparation, final approval. 4. PhD, Interdisciplinary Laboratory of Medical Research, School of Medicine, UFMG, Belo Horizonte-MG, Brasil. Conception and design of the study, acquisition of data, technical procedures, histopathological examinations, manuscript preparation, final approval. 5. PhD, Fundação Hospitalar do Estado de Minas Gerais, Belo Horizonte-MG, Brasil. Conception and design of the study, acquisition of data, technical procedures, manuscript preparation, final approval. 6. PhD, Interdisciplinary Laboratory of Medical Research, School of Medicine, UFMG, Belo Horizonte-MG, Brasil. Acquisition, analysis and interpretation of data, manuscript preparation, critical revision, final approval. 7. Full Professor, Interdisciplinary Laboratory of Medical Research, School of Medicine, UFMG, Belo Horizonte-MG, Brasil. Conception and design of the study, interpretation of data, statistical analysis, histopathological examinations, manuscript preparation and writing, critical revision, final approval.
Abstract
PURPOSE: : To characterize an experimental model of progressive renal disease induced by different degrees of nephrectomy in rats. METHODS: : Eighty male Wistar rats were divided into four experimental groups (n=20/group): sham surgery (control group), progressive degrees of nephrectomy leading to mild uremia (group 1), moderate uremia (group 2) and severe uremia (group 3). Ten animals of each group were followed for two or four weeks. At the end, blood and 24-hour urine samples were collected to determine renal function parameters. Urine output and water and food intake were daily monitored. RESULTS: : In rats of group 1, serum levels of creatinine and urea and microalbuminuria were increased, while reduced creatinine clearance (p<0.05, compared with control group), without changing blood pressure. Animals of group 2 had more accentuated alterations: increases in urinary output, blood pressure, serum concentrations of urea, creatinine, sodium, potassium, and in microalbuminuria, and reduction of creatinine clearance (p<0.05). Group 3 exhibited even more increased serum concentrations of urea, creatinine, sodium and potassium, blood pressure and microalbuminuria, and decreased creatinine clearance (p<0.05) in comparison with control group and unilateral nephrectomy. CONCLUSION: : Progressive nephrectomy in rats seems to be useful to study the physiopathology of chronic kidney disease and its mechanisms of progression.
PURPOSE: : To characterize an experimental model of progressive renal disease induced by different degrees of nephrectomy in rats. METHODS: : Eighty male Wistar rats were divided into four experimental groups (n=20/group): sham surgery (control group), progressive degrees of nephrectomy leading to mild uremia (group 1), moderate uremia (group 2) and severe uremia (group 3). Ten animals of each group were followed for two or four weeks. At the end, blood and 24-hour urine samples were collected to determine renal function parameters. Urine output and water and food intake were daily monitored. RESULTS: : In rats of group 1, serum levels of creatinine and urea and microalbuminuria were increased, while reduced creatinine clearance (p<0.05, compared with control group), without changing blood pressure. Animals of group 2 had more accentuated alterations: increases in urinary output, blood pressure, serum concentrations of urea, creatinine, sodium, potassium, and in microalbuminuria, and reduction of creatinine clearance (p<0.05). Group 3 exhibited even more increased serum concentrations of urea, creatinine, sodium and potassium, blood pressure and microalbuminuria, and decreased creatinine clearance (p<0.05) in comparison with control group and unilateral nephrectomy. CONCLUSION: : Progressive nephrectomy in rats seems to be useful to study the physiopathology of chronic kidney disease and its mechanisms of progression.
Authors: Hala M F El Miniawy; Haithem A Farghali; Marwa S Khattab; Ibrahim A Emam; Essam M Ibrahem; Dina Sabry; Tahany A Ismail Journal: Stem Cell Res Ther Date: 2022-07-30 Impact factor: 8.079
Authors: Willem B van Ham; Elise L Kessler; Marish I F J Oerlemans; M Louis Handoko; Joost P G Sluijter; Toon A B van Veen; Hester M den Ruijter; Saskia C A de Jager Journal: JACC Basic Transl Sci Date: 2022-05-25