Paulo Sandler1, Bernardo Mastella2, Diego Uchôa3, Geraldo Pereira Jotz4, Henrique Záchia Leão5, Leandro Totti Cavazzola6. 1. MD, Department of Surgery, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. Conception and design of the study, critical revision. 2. Undergraduent student, School of Medicine, HCPA, UFRGS, Porto Alegre-RS, Brazil. Acquisition, analysis and interpretation of data; technical procedures; manuscript preparation. 3. PhD, Department of Pathology, HCPA, UFRGS, Porto Alegre-RS, Brazil. Histopathological examinations, critical revision. 4. PhD, Postgraduate Program in Health Sciences, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA). Morphological Sciences Department, UFRGS, Porto Alegre-RS, Brazil. Conception and design of the study. 5. DHSc, Postgraduate Program in Health Sciences, UFCSPA. School of Medicine, Universidade Luterana do Brasil (ULBRA), Canoas-RS. Morphological Sciences Department, UFRGS, Porto Alegre-RS, Brazil. Conception and design of the study. 6. PhD, Department of Surgery, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. Scientific, intellectual, conception and design of the study; analysis and interpretation of data, critical revision, final approval.
Abstract
PURPOSE: : To analysis the effects of passive smoking on the microstructure of tissues of the abdominal wall regarding microcirculation, using histopathological study of the tobacco exposed rats. METHODS: : Twenty four male Wistar rats were divided in Control Group (CG = 8 animals) and Exposition Groups (EG = 16 animals). EG was exposed to cigarette smoke 4x/day for 120 days, while CG was preserved from exposure. Food, water and housing were similar for both groups. After 120 days, urine samples were collected before necropsy to analyze cotinine levels (ng/mL) in urine and blinded histopathological analysis of the abdominal wall performed to count arteries and veins in dermal and muscular fascia layer. RESULTS: : No difference in weight was observed between both groups (P>0.05). Cotinine concentration was significantly higher in EG (P<0.05). In dermal layer, the average of vessels per animal was 8.72 (IC95%: 8.31-9.13) for CG and 11,23 (IC95%: 10.09-12.38) for EG. In muscular fascia layer the average of vessels per animal was 17.97 (IC95%: 15.79-20.15) for CG, whereas the average for EG was of 14,85 (IC95%: 12.71-17.01) (P<0.05). CONCLUSION: : Exposition to passive smoking may cause increase in the number of vessels in dermal layer, with the opposite effects at the muscular fascia layer.
PURPOSE: : To analysis the effects of passive smoking on the microstructure of tissues of the abdominal wall regarding microcirculation, using histopathological study of the tobacco exposed rats. METHODS: : Twenty four male Wistar rats were divided in Control Group (CG = 8 animals) and Exposition Groups (EG = 16 animals). EG was exposed to cigarette smoke 4x/day for 120 days, while CG was preserved from exposure. Food, water and housing were similar for both groups. After 120 days, urine samples were collected before necropsy to analyze cotinine levels (ng/mL) in urine and blinded histopathological analysis of the abdominal wall performed to count arteries and veins in dermal and muscular fascia layer. RESULTS: : No difference in weight was observed between both groups (P>0.05). Cotinine concentration was significantly higher in EG (P<0.05). In dermal layer, the average of vessels per animal was 8.72 (IC95%: 8.31-9.13) for CG and 11,23 (IC95%: 10.09-12.38) for EG. In muscular fascia layer the average of vessels per animal was 17.97 (IC95%: 15.79-20.15) for CG, whereas the average for EG was of 14,85 (IC95%: 12.71-17.01) (P<0.05). CONCLUSION: : Exposition to passive smoking may cause increase in the number of vessels in dermal layer, with the opposite effects at the muscular fascia layer.