Literature DB >> 27980494

In Vivo Osteoinduction: Evaluating 2-Beta Coxatene as an Immunoinductive Compound and Novel Ingredient for Joint Support.

Katherine Spinks1, James J Scaffidi1.   

Abstract

CONTEXT: Osteoarthritis (OA) is a degenerative joint disease characterized by progressive loss of articular cartilage. Many treatments lack the ability to stimulate the growth of native cartilage tissue while they simultaneously increase joint comfort. For the past few decades, dietary supplements have been investigated for the ability to both address joint inflammation and stimulate cartilage tissue.
OBJECTIVES: The present study intended to examine the supplement's in vivo osteoinductive capabilities and clinical efficacy for overall joint health.
DESIGN: The research team designed a randomized, double-blind, comparative clinical trial.
SETTING: The study took place via telephone interviews. PARTICIPANTS: Participants had self-reported OA of a weight-bearing joint (ie, of the knee, hip, spine, or ankle). Patients were recruited using the Health Science Institute, a consumer supplement newsletter. INTERVENTION: Participants in the intervention group were blindly given 135 mg of 2-Beta Coxatene (2BCT) orally, which contained (1) a custom blend of low-dose Cyplexinol, an osteoinductive protein complex derived from bovine bone tissue, and (2) Boswellia serrata resin enriched to 65% 3-O-Acetyl-11-keto-β-Boswellic acid. A positive control group was blindly given 1500 mg of glucosamine hydrogen chloride and 1200 mg of chondroitin sulfate. Participants took the supplements for 3 mo. OUTCOME MEASURES: A histological evaluation was performed on an athymic rat to test the supplement's in vivo osteoinductive capabilities. A negative control, commercially purchased, unhydrolyzed type 2 collagen was used for that test. Participants were evaluated for parameters of pain and joint function at baseline (day 0) and at days 7, 30, and 90 using the Western Ontario and McMaster Universities (WOMAC) OA index and a visual analogue scale (VAS).
RESULTS: The histological evaluation of the athymic rat confirmed that the Cyplexinol component of the 2BCT was positive for de novo bone tissue and collagen synthesis, corroborating osteoinduction. In the clinical trial, the intervention group reported significant decreases of 57.4%, 52.5%, and 58% in normalized WOMAC scores for pain, stiffness, and joint functionality, respectively, from baseline to postintervention. The control group reported a decrease of 17.5%, 18.1%, and 23.9% for pain, stiffness, and joint functionality, respectively. For the intervention group, pain intensity and frequency, as measured by the VAS, also decreased 57.1% and 56.3%, respectively, from baseline to postintervention, whereas the control group showed a decrease in VAS scores of 18.0% and 14.8%, respectively. In total, an average of 81.2% of participants administered the 2BCT had reported a statistically significant improvement from baseline to postintervention, compared with 22.9% of participants administered glucosamine and chondroitin.
CONCLUSIONS: In vivo studies confirmed that the bioactive proteins (Cyplexinol) within the 2BCT stimulated de novo bone and cartilage tissue production, demonstrating osteoinduction. The intervention group reported greater improvements in the psychometric evaluations that assessed joint comfort when compared with participants given the glucosamine and chondroitin. The results suggest that 2BCT may provide a novel and synergistic response to preserving joint homeostasis and improving quality of life.

Entities:  

Year:  2016        PMID: 27980494      PMCID: PMC5145012     

Source DB:  PubMed          Journal:  Integr Med (Encinitas)        ISSN: 1546-993X


  45 in total

Review 1.  A systematic review of recommendations and guidelines for the management of osteoarthritis: The chronic osteoarthritis management initiative of the U.S. bone and joint initiative.

Authors:  Amanda E Nelson; Kelli D Allen; Yvonne M Golightly; Adam P Goode; Joanne M Jordan
Journal:  Semin Arthritis Rheum       Date:  2013-12-04       Impact factor: 5.532

Review 2.  Prevention of Renal Complications Induced by Non- Steroidal Anti-Inflammatory Drugs.

Authors:  Sonja Vuč Ković; Katarina Savić Vujović; Dragana Srebro; Branislava Medić; Tatjana Ilic-Mostic
Journal:  Curr Med Chem       Date:  2016       Impact factor: 4.530

Review 3.  Boswellia serrata: an overall assessment of in vitro, preclinical, pharmacokinetic and clinical data.

Authors:  Mona Abdel-Tawab; Oliver Werz; Manfred Schubert-Zsilavecz
Journal:  Clin Pharmacokinet       Date:  2011-06       Impact factor: 6.447

Review 4.  Nutraceuticals in the management of osteoarthritis : a critical review.

Authors:  Ryan L Ragle; Allen D Sawitzke
Journal:  Drugs Aging       Date:  2012-09       Impact factor: 3.923

5.  Relation of dietary intake and serum levels of vitamin D to progression of osteoarthritis of the knee among participants in the Framingham Study.

Authors:  T E McAlindon; D T Felson; Y Zhang; M T Hannan; P Aliabadi; B Weissman; D Rush; P W Wilson; P Jacques
Journal:  Ann Intern Med       Date:  1996-09-01       Impact factor: 25.391

6.  Bone morphogenetic protein-7 inhibits constitutive and interleukin-1 beta-induced monocyte chemoattractant protein-1 expression in human mesangial cells: role for JNK/AP-1 pathway.

Authors:  Myung-Ja Lee; Chul Woo Yang; Dong Chan Jin; Yoon Sik Chang; Byung Kee Bang; Yong-Soo Kim
Journal:  J Immunol       Date:  2003-03-01       Impact factor: 5.422

Review 7.  Osteoarthritis: diagnosis and therapeutic considerations.

Authors:  Ralph Hinton; Ron L Moody; Alan W Davis; Sean F Thomas
Journal:  Am Fam Physician       Date:  2002-03-01       Impact factor: 3.292

Review 8.  Difficulties with assessing the benefit of glucosamine sulphate as a treatment for osteoarthritis.

Authors:  Nikki Burdett; Julian David McNeil
Journal:  Int J Evid Based Healthc       Date:  2012-09

9.  Combined chondroitin sulfate and glucosamine for painful knee osteoarthritis: a multicentre, randomised, double-blind, non-inferiority trial versus celecoxib.

Authors:  Marc C Hochberg; Johanne Martel-Pelletier; Jordi Monfort; Ingrid Möller; Juan Ramón Castillo; Nigel Arden; Francis Berenbaum; Francisco J Blanco; Philip G Conaghan; Gema Doménech; Yves Henrotin; Thomas Pap; Pascal Richette; Allen Sawitzke; Patrick du Souich; Jean-Pierre Pelletier
Journal:  Ann Rheum Dis       Date:  2015-01-14       Impact factor: 19.103

10.  A double blind, randomized, placebo controlled study of the efficacy and safety of 5-Loxin for treatment of osteoarthritis of the knee.

Authors:  Krishanu Sengupta; Krishnaraju V Alluri; Andey Rama Satish; Simanchala Mishra; Trimurtulu Golakoti; Kadainti Vs Sarma; Dipak Dey; Siba P Raychaudhuri
Journal:  Arthritis Res Ther       Date:  2008-07-30       Impact factor: 5.156

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