Draft Genome Sequence of Streptococcus anginosus BVI, a New Vaginal Pathogen Candidate.

Streptococcus anginosus is a pathogen implicated in urogenital and gastroinstestinal tract infections. Here, we report the draft genome sequence of S. anginosus BVI, isolated from a bacterial vaginosis patient attending a prenatal care unit in Cali, Colombia. The genome sequence of BVI consists of 2,014,025 bp, encoding 2,008 predicted proteins.

GENOME ANNOUNCEMENT

Streptococcus anginosus and related streptococci have been primarily recognized as commensals of the human mucosa (1), since the full appreciation of their clinical significance was hampered for a long time due to difficulties in correct species identification (2, 3). In recent years, members of the S. anginosus group have been detected as potential pathogens in abscesses and blood cultures, and they also play a role in cystic fibrosis (1, 4). In other studies, S. anginosus has been recognized as an associated pathogen of different body sites, such as the oral cavity and the urogenital and gastroinstestinal tracts (5, 6). Although S. anginosus has been found in the human vaginal and urinary microbiome (7–10), a genome of an S. anginosus isolate found in the vaginal canal has not been sequenced so far.

Here, we report the draft genome sequence of S. anginosus BVI that was isolated from the vaginal fluid of a pregnant patient with bacterial vaginosis attending routine prenatal care. The respective specimen was collected with a sterile swab and cultured on human blood Tween (HBT) agar in 5% CO2. Colonies were subcultured on HBT agar, and genomic DNA was purified according to a salt-based miniscale protocol (11). The genomic DNA was sequenced by the paired-end technique on the MiSeq system (Illumina). The sequencing run yielded 699,061 reads accounting for ~203 Mb of total DNA information. In this way, an approximately 100-fold coverage was achieved for the 2.0-Mbp genome of S. anginosus BVI. The assembly was carried out with the GS De Novo Assembler software (version 2.8). The draft genome sequence consists of 26 contigs with a total size of 2,014,025 bp and a G+C content of 38.9%. Pairwise DNA comparisons with the complete genome sequences of S. anginosus strains C238 (accession no. CP003861), SA1 (accession no. CP007573), and J4211 (accession no. CP012805) by average nucleotide identity (ANI) analysis (12) revealed values above 95% for each genome pair, confirming the taxonomic classification of the BVI isolate as S. anginosus.

The annotation of the draft genome sequence was performed with the Prokka version 1.11 software (13) and the GenDB 2.4 platform (14)). The draft genome sequence of S. anginosus BVI contains 2,008 protein-coding regions and 46 tRNA genes. As S. anginosus BVI was isolated from a patient diagnosed with bacterial vaginosis, the exploration of virulence factors is of importance to understand bacterium-host interactions and potential virulence mechanisms. Analysis of the genome data by using the virulence factor database VFDB (15) presented a significant load of iron uptake systems classified as nonspecific virulence factors and of secretion systems classifed as offensive virulence factors. S. anginosus BVI probably represents a new vaginal pathogen that can be isolated from cases of bacterial vaginosis.

Accession number(s).

The S. anginosus BVI whole-genome shotgun project has been deposited in ENA under the accession no. FMKB00000000. The version described in this paper is the first version, FMKB01000000.