| Literature DB >> 27979071 |
Yang Xu1, Yuting Li2, Tao Bao2, Xiaodong Zheng2, Wei Chen3, Jianxu Wang4.
Abstract
Cauliflower by-products (CBP) are rich in leaf protein. Every year tons of CBP will lead to environmental pollution. Therefore, this study was conducted to extract leaf protein from CBP and investigate its biological activities. Our results showed that the optimal extraction parameters were: a liquid to solid ratio of 4mL/g, a pH of 11, an ultrasonic extraction lasting 15min, and at an applied power of 175W. Under these optimized conditions, 12.066g of soluble leaf protein (SLP) was obtained from 1000g of CBP and its extraction yield was 53.07%. The obtained SLP was further hydrolysed by Alcalase and the SLP hydrolysate (SLPH) showed a potent angiotensin I-converting enzyme (ACE) inhibitory activity with an IC50 value of 138.545μg/mL in vitro. In addition, SLPH promoted the glucose consumption and enhanced the glycogen content in HepG2 cells. Overall, our results suggested that CBP may be recycled for designing future functional foods.Entities:
Keywords: ACE inhibitory activity; Cauliflower by-products; Glucose consumption; Glycogen content; Leaf protein; Ultrasonic-assisted extraction
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Year: 2016 PMID： 27979071 DOI： 10.1016/j.foodchem.2016.10.053
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514