BACKGROUND: The objective of this study was to compare the analysis of 17-hydroxyprogesterone (17-OHP) by radio-immunoassay (RIA) in serum with analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) on dried blood spot samples (DBSS) for monitoring therapy in children with congenital adrenal hyperplasia (CAH), and to investigate differences in 17-OHP values during the day. METHODS: Fourteen children (8 females), median age 4.2 (0.3-16.0) years, were studied. Serum samples and DBSS were drawn before hydrocortisone dosing. RESULTS: 17-OHP by LC-MS/MS in DBSS were highly correlated to 17-OHP by RIA in serum, r=0.956, p<0.01. A total of 26 three-time-point series were investigated. Using only the afternoon 17-OHP values to determine the hydrocortisone doses would have led to overdosing seven times and underdosing six times. CONCLUSIONS: Good agreement was demonstrated between 17-OHP determination by RIA in serum and LC-MS/MS on DBSS. Multiple 17-OHP measurements per day are required to ensure sufficient hydrocortisone dose adjustment.
BACKGROUND: The objective of this study was to compare the analysis of 17-hydroxyprogesterone (17-OHP) by radio-immunoassay (RIA) in serum with analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) on dried blood spot samples (DBSS) for monitoring therapy in children with congenital adrenal hyperplasia (CAH), and to investigate differences in 17-OHP values during the day. METHODS: Fourteen children (8 females), median age 4.2 (0.3-16.0) years, were studied. Serum samples and DBSS were drawn before hydrocortisone dosing. RESULTS:17-OHP by LC-MS/MS in DBSS were highly correlated to 17-OHP by RIA in serum, r=0.956, p<0.01. A total of 26 three-time-point series were investigated. Using only the afternoon 17-OHP values to determine the hydrocortisone doses would have led to overdosing seven times and underdosing six times. CONCLUSIONS: Good agreement was demonstrated between 17-OHP determination by RIA in serum and LC-MS/MS on DBSS. Multiple 17-OHP measurements per day are required to ensure sufficient hydrocortisone dose adjustment.
Authors: Mariska A M Schröder; Antonius E van Herwaarden; Paul N Span; Erica L T van den Akker; Gianni Bocca; Sabine E Hannema; Hetty J van der Kamp; Sandra W K de Kort; Christiaan F Mooij; Dina A Schott; Saartje Straetemans; Vera van Tellingen; Janiëlle A van der Velden; Fred C G J Sweep; Hedi L Claahsen-van der Grinten Journal: J Clin Endocrinol Metab Date: 2022-03-24 Impact factor: 5.958
Authors: Bas P H Adriaansen; Johannes S Kamphuis; Mariska A M Schröder; André J Olthaar; Carina Bock; André Brandt; Nike M M L Stikkelbroeck; Eef G W M Lentjes; Paul N Span; Fred C G J Sweep; Hedi L Claahsen-van der Grinten; Antonius E van Herwaarden Journal: Clin Endocrinol (Oxf) Date: 2022-02-23 Impact factor: 3.523