| Literature DB >> 27975321 |
Li-Ling Wu1, Hurng-Yi Wang1, Pei-Jer Chen2.
Abstract
Hydrodynamic HBV transfection mouse model is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.Entities:
Keywords: Gene transfer; HBV persistence; Hydrodynamic injection; In vivo transfection; Liver tolerance
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Year: 2017 PMID： 27975321 DOI： 10.1007/978-1-4939-6700-1_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745