Literature DB >> 27975317

Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay.

Ran Yan1, Dawei Cai1, Yuanjie Liu1, Haitao Guo2.   

Abstract

The culture fluid of HBV replicating cells contains a mixture of viral particles with different structural and genetic components, including enveloped infectious virions, genome-free virion, envelope-only subviral particles, and nonenveloped naked capsids. Based on their different physical and chemical properties, the enveloped and nonenveloped particles can be separated by the native agarose gel electrophoresis and transferred onto a positively charged microporous membrane, then the virus particle-associated protein components and nucleic acid content can be detected by antibody-based enzyme immunoassay (EIA) and hybridization, respectively. Such convenient experimental procedure is called HBV particle assay and described in detail in this chapter. The particle gel assay can be used to study viral and host regulations of HBV virus morphogenesis and egress, and for antiviral assessment of HBV inhibitors as well.

Entities:  

Keywords:  HBV particles; Particle gel assay

Mesh：

Substances：

Year:  2017        PMID： 27975317      PMCID： PMC5642045          DOI： 10.1007/978-1-4939-6700-1_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

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Authors:  J Summers; A O'Connell; I Millman
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

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3.  Hepatitis B small surface antigen particles are octahedral.

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Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-03       Impact factor: 11.205

4.  Coordinate regulation of replication and virus assembly by the large envelope protein of an avian hepadnavirus.

Authors:  R J Lenhoff; J Summers
Journal:  J Virol       Date:  1994-07       Impact factor: 5.103

5.  Alkylated porphyrins have broad antiviral activity against hepadnaviruses, flaviviruses, filoviruses, and arenaviruses.

Authors:  Haitao Guo; Xiaoben Pan; Richeng Mao; Xianchao Zhang; Lijuan Wang; Xuanyong Lu; Jinhong Chang; Ju-Tao Guo; Shendra Passic; Fred C Krebs; Brian Wigdahl; Travis K Warren; Cary J Retterer; Sina Bavari; Xiaodong Xu; Andrea Cuconati; Timothy M Block
Journal:  Antimicrob Agents Chemother       Date:  2010-12-06       Impact factor: 5.191

6.  Intracellular assembly and packaging of hepatitis B surface antigen particles occur in the endoplasmic reticulum.

Authors:  E J Patzer; G R Nakamura; C C Simonsen; A D Levinson; R Brands
Journal:  J Virol       Date:  1986-06       Impact factor: 5.103

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Authors:  C Possehl; R Repp; K H Heermann; E Korec; A Uy; W H Gerlich
Journal:  Arch Virol Suppl       Date:  1992

8.  Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin.

Authors:  Carsten Lambert; Tatjana Döring; Reinhild Prange
Journal:  J Virol       Date:  2007-06-06       Impact factor: 5.103

9.  Involvement of host cellular multivesicular body functions in hepatitis B virus budding.

Authors:  Tokiko Watanabe; Ericka M Sorensen; Akira Naito; Meghan Schott; Seungtaek Kim; Paul Ahlquist
Journal:  Proc Natl Acad Sci U S A       Date:  2007-06-05       Impact factor: 11.205

10.  The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.

Authors:  Shu-Fan Chou; Ming-Lin Tsai; Jyun-Yuan Huang; Ya-Shu Chang; Chiaho Shih
Journal:  PLoS Pathog       Date:  2015-10-02       Impact factor: 6.823
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  6 in total

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Authors:  Yongmei Zhang; Hu Zhang; Junjie Zhang; Jiming Zhang; Haitao Guo
Journal:  Antiviral Res       Date:  2019-03-19       Impact factor: 5.970

2.  Hepatitis B Virus Precore Protein p22 Inhibits Alpha Interferon Signaling by Blocking STAT Nuclear Translocation.

Authors:  Bidisha Mitra; Jinyu Wang; Elena S Kim; Richeng Mao; Minhui Dong; Yuanjie Liu; Jiming Zhang; Haitao Guo
Journal:  J Virol       Date:  2019-06-14       Impact factor: 5.103

3.  Biogenesis and molecular characteristics of serum hepatitis B virus RNA.

Authors:  Sheng Shen; Zhanglian Xie; Dawei Cai; Xiaoyang Yu; Hu Zhang; Elena S Kim; Bin Zhou; Jinlin Hou; Xiaoyong Zhang; Qi Huang; Jian Sun; Haitao Guo
Journal:  PLoS Pathog       Date:  2020-10-20       Impact factor: 6.823

4.  Structure-Based Discovery of N-Sulfonylpiperidine-3-Carboxamides as Novel Capsid Assembly Modulators for Potent Inhibition of HBV Replication.

Authors:  Yang Yang; Yu Yan; Jiaxin Yin; Jie Hu; Xuefei Cai; Jieli Hu; Jie Xia; Kai Wang; Ni Tang; Luyi Huang
Journal:  Viruses       Date:  2022-02-08       Impact factor: 5.048

5.  Immunomolecular assay based on selective virion capture by spike antibody and viral nucleic acid amplification for detecting intact SARS-CoV-2 particles.

Authors:  Xiaoli Wu; Junye Liu; Hongpeng Zhang; Hua Zhou; Wen Wang; Yuanyan Ma; Shimei Shen; Xuefei Cai; Ailong Huang; Deqiang Wang
Journal:  J Nanobiotechnology       Date:  2022-09-05       Impact factor: 9.429

6.  Elevated NTCP expression by an iPSC-derived human hepatocyte maintenance medium enhances HBV infection in NTCP-reconstituted HepG2 cells.

Authors:  Xinlei Li; Zhaohui Xu; Bidisha Mitra; Minghang Wang; Haitao Guo; Zongdi Feng
Journal:  Cell Biosci       Date:  2021-07-05       Impact factor: 7.133
  6 in total

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