Literature DB >> 27975309

Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation-Specific PCR.

Yongmei Zhang1, Richeng Mao1, Haitao Guo2, Jiming Zhang3.   

Abstract

Mapping of DNA methylation is essential in understanding the process of HBV covalently closed circular DNA (cccDNA) transcription. Here, bisulfite sequencing PCR and methylation-specific PCR, two PCR-based approaches used in determining and quantifying the DNA methylation pattern, are described.

Entities:  

Keywords:  Bisulfite sequencing PCR; DNA methylation; HBV cccDNA; Methylation-specific PCR

Mesh：

Substances：

Year:  2017        PMID： 27975309      PMCID： PMC5642044          DOI： 10.1007/978-1-4939-6700-1_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

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Authors:  P A Jones; D Takai
Journal:  Science       Date:  2001-08-10       Impact factor: 47.728

2.  Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.

Authors:  J Summers; A O'Connell; I Millman
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

Review 3.  Hepatitis B virus biology.

Authors:  C Seeger; W S Mason
Journal:  Microbiol Mol Biol Rev       Date:  2000-03       Impact factor: 11.056

4.  Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription.

Authors:  P L Jones; G J Veenstra; P A Wade; D Vermaak; S U Kass; N Landsberger; J Strouboulis; A P Wolffe
Journal:  Nat Genet       Date:  1998-06       Impact factor: 38.330

5.  MethPrimer: designing primers for methylation PCRs.

Authors:  Long-Cheng Li; Rajvir Dahiya
Journal:  Bioinformatics       Date:  2002-11       Impact factor: 6.937

6.  Replicative activity of hepatitis B virus is negatively associated with methylation of covalently closed circular DNA in advanced hepatitis B virus infection.

Authors:  Jin-Wook Kim; Sang Hyub Lee; Young Soo Park; Jin-Hyeok Hwang; Sook-Hyang Jeong; Nayoung Kim; Dong Ho Lee
Journal:  Intervirology       Date:  2011-01-14       Impact factor: 1.763

7.  Evidence that methylation of hepatitis B virus covalently closed circular DNA in liver tissues of patients with chronic hepatitis B modulates HBV replication.

Authors:  Yanhai Guo; Yongnian Li; Shijie Mu; Ju Zhang; Zhen Yan
Journal:  J Med Virol       Date:  2009-07       Impact factor: 2.327

8.  The covalently closed duplex form of the hepadnavirus genome exists in situ as a heterogeneous population of viral minichromosomes.

Authors:  J E Newbold; H Xin; M Tencza; G Sherman; J Dean; S Bowden; S Locarnini
Journal:  J Virol       Date:  1995-06       Impact factor: 5.103

9.  CpG-methylation regulates a class of Epstein-Barr virus promoters.

Authors:  Martin Bergbauer; Markus Kalla; Anne Schmeinck; Christine Göbel; Ulrich Rothbauer; Sebastian Eck; Anna Benet-Pagès; Tim M Strom; Wolfgang Hammerschmidt
Journal:  PLoS Pathog       Date:  2010-09-23       Impact factor: 6.823

10.  Comprehensive DNA methylation analysis of hepatitis B virus genome in infected liver tissues.

Authors:  Surbhi Jain; Ting-Tsung Chang; Sitong Chen; Batbold Boldbaatar; Adam Clemens; Selena Y Lin; Ran Yan; Chi-Tan Hu; Haitao Guo; Timothy M Block; Wei Song; Ying-Hsiu Su
Journal:  Sci Rep       Date:  2015-05-22       Impact factor: 4.379
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  4 in total

1.  Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered iNOS gene aberrant methylation.

Authors:  Zhen-Yu Gong; Zhi-Qiang Yuan; Zhi-Wei Dong; Yi-Zhi Peng
Journal:  Am J Transl Res       Date:  2017-05-15       Impact factor: 4.060

2.  An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes.

Authors:  Yutaka Furutani; Mariko Toguchi; Yumi Shiozaki-Sato; Xian-Yang Qin; Etsuko Ebisui; Shoko Higuchi; Masayuki Sudoh; Harukazu Suzuki; Nobuaki Takahashi; Koichi Watashi; Takaji Wakita; Hideaki Kakeya; Soichi Kojima
Journal:  PLoS One       Date:  2019-06-12       Impact factor: 3.240

Review 3.  Clinical Implications of Hepatitis B Virus RNA and Covalently Closed Circular DNA in Monitoring Patients with Chronic Hepatitis B Today with a Gaze into the Future: The Field Is Unprepared for a Sterilizing Cure.

Authors:  Anastasiya Kostyusheva; Dmitry Kostyushev; Sergey Brezgin; Elena Volchkova; Vladimir Chulanov
Journal:  Genes (Basel)       Date:  2018-10-05       Impact factor: 4.096

4.  Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS.

Authors:  Yibin Liu; Jingfei Cheng; Paulina Siejka-Zielińska; Carika Weldon; Hannah Roberts; Maria Lopopolo; Andrea Magri; Valentina D'Arienzo; James M Harris; Jane A McKeating; Chun-Xiao Song
Journal:  Genome Biol       Date:  2020-03-03       Impact factor: 13.583
  4 in total

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