Literature DB >> 27975308

Analyses of HBV cccDNA Quantification and Modification.

Yuchen Xia1, Daniela Stadler1, Chunkyu Ko1, Ulrike Protzer2.   

Abstract

Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

Entities:  

Keywords:  Covalently closed circular DNA; Deamination; Differential DNA denaturation PCR; Quantitative PCR; Quantitative differential DNA denaturation PCR

Mesh：

Substances：

Year:  2017        PMID： 27975308     DOI： 10.1007/978-1-4939-6700-1_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  Differences in HBV Replication, APOBEC3 Family Expression, and Inflammatory Cytokine Levels Between Wild-Type HBV and Pre-core (G1896A) or Basal Core Promoter (A1762T/G1764A) Mutants.

Authors:  Keith C K Lau; Shivali S Joshi; Douglas J Mahoney; Andrew L Mason; Guido van Marle; Carla Osiowy; Carla S Coffin
Journal:  Front Microbiol       Date:  2020-07-14       Impact factor: 5.640

2.  A New Role for Capsid Assembly Modulators To Target Mature Hepatitis B Virus Capsids and Prevent Virus Infection.

Authors:  Chunkyu Ko; Romina Bester; Xue Zhou; Zhiheng Xu; Christoph Blossey; Julia Sacherl; Florian W R Vondran; Lu Gao; Ulrike Protzer
Journal:  Antimicrob Agents Chemother       Date:  2019-12-20       Impact factor: 5.191

Review 3.  New directions in hepatitis B therapy research.

Authors:  Marta Lewandowska; Anna Piekarska
Journal:  Clin Exp Hepatol       Date:  2017-07-05

4.  PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core.

Authors:  Barbora Lubyova; Jan Hodek; Ales Zabransky; Hana Prouzova; Martin Hubalek; Ivan Hirsch; Jan Weber
Journal:  PLoS One       Date:  2017-10-24       Impact factor: 3.240

Review 5.  Detection of HBV Covalently Closed Circular DNA.

Authors:  Xiaoling Li; Jinghua Zhao; Quan Yuan; Ningshao Xia
Journal:  Viruses       Date:  2017-06-06       Impact factor: 5.048

Review 6.  A Role for the Host DNA Damage Response in Hepatitis B Virus cccDNA Formation-and Beyond?

Authors:  Sabrina Schreiner; Michael Nassal
Journal:  Viruses       Date:  2017-05-22       Impact factor: 5.048

7.  One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors.

Authors:  Maren Schiwon; Eric Ehrke-Schulz; Andreas Oswald; Thorsten Bergmann; Thomas Michler; Ulrike Protzer; Anja Ehrhardt
Journal:  Mol Ther Nucleic Acids       Date:  2018-06-07       Impact factor: 8.886

8.  Hepatitis B virus genome recycling and de novo secondary infection events maintain stable cccDNA levels.

Authors:  Chunkyu Ko; Anindita Chakraborty; Wen-Min Chou; Julia Hasreiter; Jochen M Wettengel; Daniela Stadler; Romina Bester; Theresa Asen; Ke Zhang; Karin Wisskirchen; Jane A McKeating; Wang-Shick Ryu; Ulrike Protzer
Journal:  J Hepatol       Date:  2018-08-22       Impact factor: 25.083

9.  Cas9-targeted nanopore sequencing reveals epigenetic heterogeneity after de novo assembly of native full-length hepatitis B virus genomes.

Authors:  Chloe Goldsmith; Damien Cohen; Anaëlle Dubois; Maria Guadalupe Martinez; Kilian Petitjean; Anne Corlu; Barbara Testoni; Hector Hernandez-Vargas; Isabelle Chemin
Journal:  Microb Genom       Date:  2021-05

10.  IFN-α-mediated Base Excision Repair Pathway Correlates with Antiviral Response Against Hepatitis B Virus Infection.

Authors:  Yong Li; Yuchen Xia; Meifang Han; Guang Chen; Dake Zhang; Wolfgang E Thasler; Ulrike Protzer; Qin Ning
Journal:  Sci Rep       Date:  2017-10-05       Impact factor: 4.379
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